Kemptide
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Kemptide is a synthetic heptapeptide that acts as a substrate for cAMP-dependent protein kinase (PK) [1].
Cyclic AMP-dependent protein kinase is the sole intracellular acceptor for cyclic AMP in all cell types. Protein phosphorylation by different types of protein kinase (PK) activities comprises a form of protein modification that plays an important role in the regulation of numerous biological processes [1][2].
Kemptide is a novel synthetic heptapeptide phosphate acceptor that acts as a substrate for cAMP-dependent protein kinase. Kemptide had all the properties of a cytophilic substrate and could preserving cell membrane intactness when added to cultured cells. In the presence of extracellular ATP and cAMP, different types of intact cells catalyzed the phosphorylation of Kemptide with Km values of 3-4 μM. Kemptide phosphorylation was influenced by PKI, the inhibitory protein specific for cAMP-PK [1]. Kemptide was usually used as a substrate for detection of activities of soluble A-kinase isolated from guinea-pig lung parenchyma and from bovine tracheal smooth muscle [2][3].
References:
[1]. Kübler D, Pyerin W, Bill O, et al. Evidence for ecto-protein kinase activity that phosphorylates Kemptide in a cyclic AMP-dependent mode. J Biol Chem. 1989 Aug 25;264(24):14549-55.
[2]. Giembycz MA, Diamond J. Evaluation of kemptide, a synthetic serine-containing heptapeptide, as a phosphate acceptor for the estimation of cyclic AMP-dependent protein kinase activity in respiratory tissues. Biochem Pharmacol. 1990 Jan 15;39(2):271-83.
[3]. Langlands JM, Rodger IW. Determination of soluble cAMP-dependent protein kinase activity in guinea-pig tracheal smooth muscle. Preferential use of kemptide as a phosphorylating substrate. Biochem Pharmacol. 1990 Apr 15;39(8):1365-74.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 771.9 |
Cas No. | 65189-71-1 |
Formula | C32H61N13O9 |
Synonyms | NSC 332190 |
Solubility | Soluble in DMSO |
Chemical Name | (5S,8S,11S,14S,17S,20S)-20-amino-14,17-bis(3-guanidinopropyl)-8-(hydroxymethyl)-5-isobutyl-11,22-dimethyl-4,7,10,13,16,19-hexaoxo-3,6,9,12,15,18-hexaazatricosan-1-oic acid |
SDF | Download SDF |
Canonical SMILES | OC(CNC([C@H](CC(C)C)NC([C@H](CO)NC([C@H](C)NC([C@@H](NC([C@@H](NC([C@@H](N)CC(C)C)=O)CCCNC(N)=N)=O)CCCNC(N)=N)=O)=O)=O)=O)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
Quality Control & MSDS
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Purity ≥ 95.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)