Jasplakinolide
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Jasplakinolide, a cyclodepsipeptide originally isolated from the marine sponge, Jaspis johnstoni, is a potent inducer of actin polymerization, which also stabilizes pre-formed actin filaments. Jasplakinolide may exert its cytotoxic effect by binding to F-actin. Compared with Ca2+-actin, jasplakinolide shows a much greater effect on Mg2+-actin, and binds to F-actin competitively with phalloidin with a Kd value of ~ 15 nM. Jasplakinolide exhibits both fungicidal and antiproliferative activity. In addition, jasplakinolide is membrane-permeable, which makes it a commonly used tool in cell biology, when actin polymerization or actin filament stabilization has to be achieved.
References:
1. Bubb MR, Senderowicz AM, Sausville EA, et al. Jasplakinolide, a cytotoxic natural product, induces actin polymerization and competitively inhibits the binding of phalloidin to F-actin. Journal of Biological Chemistry, 1994, 269(21): 14869-14871.
2. Holzinger A. Jasplakinolide: an actin-specific reagent that promotes actin polymerization. Methods in Molecular Biology, 2009, 586: 71-87.
| Physical Appearance | An off- |
| Storage | Store at -20°C |
| M.Wt | 709.67 |
| Cas No. | 102396-24-7 |
| Formula | C36H45BrN4O6 |
| Solubility | Soluble in DMSO |
| Chemical Name | (4R,7R,10S,13R,17R,19S,E)-7-((2-bromo-1H-indol-3-yl)methyl)-4-(4-hydroxyphenyl)-8,10,13,15,17,19-hexamethyl-1-oxa-5,8,11-triazacyclononadec-15-ene-2,6,9,12-tetraone |
| SDF | Download SDF |
| Canonical SMILES | BrC(NC1=CC=CC=C21)=C2C[C@H](C(N[C@@H](C(C=C3)=CC=C3O)CC(O[C@@H](C)C[C@@H](C)C=C(C)C[C@@H](C)C(N[C@H]4C)=O)=O)=O)N(C)C4=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- View current batch:
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Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
化学结构
