IWP 4
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IWP 4 is an inhibitor of Wnt production. IWP 4 impairs Wnt pathway activity (IC50 = 25 nM) by inactivating Porcupine, a membrane-bound acyltransferase responsible for attaching a palmitoyl group to Wnt proteins, which is essential to Wnt signaling and Wnt secretion. The Wnt family of signaling proteins is involved in most aspects of embryonic development and postembryonic tissue homeostasis. Hyperactivation of the Wnt/β-catenin pathway induces premature senescence of stem cells and age-related loss of stem cell function. In cancer, hyperactivation of the Wnt/β-catenin pathway often exists along with mutations in other cell growth regulatory genes, which results in aberrant cell growth. Thus, IWP 4 could serve a useful tool for researching tissue regeneration and Wnt ligand-dependent tumors. IWP 4 has been used for inducing cardiomyocyte differentiation from human pluripotent stem cells.
References:
1. Chen B, Dodge ME, Tang W, et al. Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nature Chemical Biology, 2009, 5(2): 100-107.
2. Lian X, Hsiao C, Wilson G, et al. Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(27): E1848-1857.
| Physical Appearance | A crystalline solid |
| Storage | Store at -20°C |
| M.Wt | 496.62 |
| Cas No. | 686772-17-8 |
| Formula | C23H20N4O3S3 |
| Solubility | insoluble in H2O; insoluble in EtOH; insoluble in DMSO |
| Chemical Name | 2-((3-(2-methoxyphenyl)-4-oxo-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)thio)-N-(6-methylbenzo[d]thiazol-2-yl)acetamide |
| SDF | Download SDF |
| Canonical SMILES | O=C(N(C1=C(OC)C=CC=C1)C(SCC(NC2=NC(C=CC(C)=C3)=C3S2)=O)=N4)C5=C4CCS5 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Cell experiment:[1] | |
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Cell lines |
L-Wnt-STF cells that exhibit constitutive Wnt pathway activation |
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Reaction Conditions |
5 μM IWP 4 for 24 h incubation |
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Applications |
IWP 4 blocked Wnt-dependent phosphorylation of the low-density lipoprotein receptor-related protein 6 (Lrp6) receptor and the scaffold protein Dishevelled (Dvl), thereby preventing the accumulation of β-catenin. |
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Note |
The technical data provided above is for reference only. |
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References: 1. Chen B, Dodge ME, Tang W, et al. Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nature Chemical Biology, 2009, 5(2): 100-107. 2. Lian X, Hsiao C, Wilson G, et al. Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(27): E1848-1857. |
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质量控制和MSDS
- 批次:
化学结构
