HC 067047
| 规格 | 价格 | 货期 | 数量 |
|---|---|---|---|
| 10mg | ￥929.00 | 现货 | |
| 50mg | ￥3346.00 | 现货 |
特色产品
- 适用于流式细胞仪和荧光显微镜检测
- 准确区分活细胞/凋亡细胞/坏死细胞
- 用于免疫印迹和质谱分析等后续操作
- 适用于30 KDa-130 KDa大小的蛋白
- 对细胞无毒性
- 步骤更简单，无需有机溶剂
- 可将信号灵敏度提高100倍
- 同时保持稳定的特异性和分辨率
- 提供更高的转录效率并抑制免疫激活
- 使用5-moUTP和Cy5-utp修饰
- 优异的扩增效率、可重复性和稳定性
- 卓越的灵敏度和准确性，操作简单
- 优化的缓冲液和T7 RNA聚合酶混合物
- 20 μL反应体系可获得更高的RNA产量
产品描述
HC067047 is a selective TRPV4 antagonist with IC50 of 48 nM, 133 nM and 17 nM for human, rat and mouse TRPV4, respectively[1].
In HEI-OC1 cells, HC067047 (1 µM) treatment for 24 hours can significantly reduce the mRNA expression in HEI-OC1 cells cultured in high glucose and reduce the expression of TRPV4 protein. HC067047 (1 µM) treatment for 48 hours can inhibit cell proliferation and promote apoptosis[2].
In WT mice with cystiti, HC067047 (0-50 mg/kg; intraperitoneal injection; for 30 minutes) treatment increases functional bladder capacity and reduces micturition frequency. HC067047 do not affect bladder function in Trpv4−/− mice[1].
References:
[1]. Everaerts W et al. Inhibition of the cation channel TRPV4 improves bladder function in mice and rats with cyclophosphamide-induced cystitis.Proc Natl Acad Sci U S A, 2010, 107(44): 19084-9.
[2]. Xing Y, et al. Decreased Expression of TRPV4 Channels in HEI-OC1 Cells Induced by High Glucose Is Associated with Hearing Impairment. Yonsei Med J, 2018, 59(9): 1131-1137.
产品性质
| 物理外观 | A crystalline solid |
| CAS号 | 883031-03-6 |
| 分子式 | C26H28F3N3O2 |
| 分子量 | 471.51 |
| 小分子别名 | HC-067047 |
| 化学名称 | 2-methyl-1-(3-morpholinopropyl)-5-phenyl-N-(3-(trifluoromethyl)phenyl)-1H-pyrrole-3-carboxamide |
| 溶解度 | ≥47.2 mg/mL in DMSO; insoluble in H2O; ≥16.83 mg/mL in EtOH with ultrasonic |
| SMILES | Cc([n](CCCN1CCOCC1)c(-c1ccccc1)c1)c1C(Nc1cccc(C(F)(F)F)c1)=O |
| 存储条件 | -20°C |
| 运输条件 | 蓝冰 |
产品应用 (实验数据来自文献，APExBIO并未验证，仅供参考)
IC50和靶点
| 生物活性描述 | HC-067047 是一种强效的选择性 TRPV4 拮抗剂，可逆性地抑制通过人类、大鼠和小鼠 TRPV4 同源物的电流，其 IC50 值分别为 48 nM、133 nM 和 17 nM。 |
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