Granzyme B Inhibitor Z-AAD-CH2Cl
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Z-AAD-CH2Cl(Z-AAD-CMK)是一种细胞渗透性的和不可逆的颗粒酶B抑制剂[1].
颗粒酶B是一种细胞凋亡诱导因子,表达于自然杀伤(NK)细胞以及细胞毒性淋巴细胞(CTLs)颗粒和细胞毒性T细胞中,在诱导炎症和细胞外基质重塑中起着重要作用[1].
Z-AAD-CH2Cl(Z-AAD-CMK)是一种细胞渗透性的和不可逆的颗粒酶B抑制剂.在表达NK细胞表面抗原的人C-28/12软骨细胞中,Z-AAD-CMK呈剂量依赖性地抑制软骨细胞对K562细胞的细胞毒作用,该作用依赖于颗粒酶B[1].在口腔鳞状细胞癌细胞系OSC-3中,与对照组相比,Z-AAD-CMK减少白介素-2激活淋巴细胞(LAK细胞)增强的caspase-3活性(约70%),并抑制约20%的 DNA片段.与对照组相比,Z-AAD-CMK也抑制LAK细胞诱导的活性氧中间体(ROI)增加(约50%)[2].在Tc1细胞中,Z-AAD-CMK抑制细胞凋亡[3].
参考文献：
[1]. Saito S, Murakoshi K, Kotake S, et al. Granzyme B induces apoptosis of chondrocytes with natural killer cell-like cytotoxicity in rheumatoid arthritis. J Rheumatol, 2008, 35(10): 1932-1943.
[2]. Yamamoto T, Yoneda K, Ueta E, et al. Enhanced apoptosis of squamous cell carcinoma cells by interleukin-2-activated cytotoxic lymphocytes combined with radiation and anticancer drugs. Eur J Cancer, 2000, 36(15): 2007-2017.
[3]. Gorak-Stolinska P, Truman JP, Kemeny DM, et al. Activation-induced cell death of human T-cell subsets is mediated by Fas and granzyme B but is independent of TNF-alpha. J Leukoc Biol, 2001, 70(5): 756-766.
| Storage | Store at -20°C |
| M.Wt | 455.89 |
| Formula | C20H26ClN3O7 |
| Solubility | Soluble in DMSO |
| Chemical Name | (Z)-2-((E)-((benzyloxy)(hydroxy)methylene)amino)-N-((Z)-1-((5-chloro-1-methoxy-1,4-dioxopentan-3-yl)imino)-1-hydroxypropan-2-yl)propanimidic acid |
| SDF | Download SDF |
| Canonical SMILES | CC(/N=C(O)/C(/N=C(OCC1=CC=CC=C1)\O)C)/C(O)=N/C(C(CCl)=O)CC(OC)=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
化学结构
