GPBAR-A
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
GPBAR-A是胆汁酸受体GPBAR1的激动剂 [1].
G蛋白耦联胆汁酸受体1(GPBAR1,TGR5)是一种胆汁酸的血浆膜结合受体。TGR5参与胆结石的形成,并表达于人类胆囊上皮细胞[2]。
GPBAR-A是一种胆汁酸受体GPBAR1的激动剂。在GLUTag细胞中,GPBAR-A可刺激胰高血糖素样肽(GLP-1)的释放。在原代结肠培养物中,GPBAR-A可增加4.2倍的GLP-1释放。在上部小肠培养物中,GPBAR-A可增加2.6倍的GLP-1释放。GPBAR-A也可使GLUTag细胞中的cAMP浓度增加57%。通过监测细胞中340/380 nm荧光比率发现,GPBAR-A可增加56/149细胞中Ca2+信号,平均信号强度增加1.3倍。在葡萄糖存在时,GPBAR-A可使148/149细胞中Ca2+信号的平均信号强度增加2.6倍。在diazoxide(KATP通道开放剂,340 μM)和70 mM KCl存在时,GPBAR-A也增加GLP-1分泌。在结肠和小肠培养物中,GPBAR-A可分别增加2.4倍和1.5倍的GLP-1分泌。GPBAR-A介导的GLP-1分泌不依赖于KATP通道的关闭[1]。
GPBAR-A作用于结肠和小肠细胞,可使GLP-1的分泌量分别增加2.4倍和1.5倍。GPBAR-A对GLP-1的调节作用依赖于KATP通道的闭合[1]。
参考文献:
[1] Parker HE, Wallis K, le Roux CW, et al. Molecular mechanisms underlying bile acid-stimulated glucagon-like peptide-1 secretion. Br J Pharmacol, 2012, 165(2): 414-423.
[2] Keitel V, Cupisti K, Ullmer C, et al. The membrane-bound bile acid receptor TGR5 is localized in the epithelium of human gallbladders. Hepatology, 2009, 50(3): 861-870.
Physical Appearance | Off-white solid |
Storage | Store at -20°C |
M.Wt | 484.37 |
Cas No. | 877052-79-4 |
Formula | C23H15F7N2O2 |
Solubility | <9.69mg/ml in ethanol; <48.44mg/ml in DMSO |
SDF | Download SDF |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |