GnRH Associated Peptide (GAP) (1-13), human

序列：Asp-Ala-Glu-Asn-Leu-Ile-Asp-Ser-Phe-Gln-Glu-Ile-Val

将编码人促性腺激素释放激素（GnRH）的前体蛋白的cDNA序列构建进表达载体转入细菌中，在细菌中合成含有56个氨基酸的GnRH相关肽（GAP）。GAP是催乳素分泌的有效抑制剂，可以刺激促性腺激素的释放。对GAP序列相关多肽的主动免疫可导致催乳素分泌的增加。

促性腺激素释放激素（GnRH）在脊椎动物繁殖过程中发挥重要作用。从下丘脑神经元释放的十肽控制体内促性腺激素水平，其反过来调节性腺状态。促性腺激素释放激素（GnRH）在脊椎动物中广泛表达并具有保守性，显示了它的重要性。此外，促性腺激素释放激素相关肽（GAP）与GnRH共同分泌释放进入垂体门静脉血液。

参考文献：
1.  Károly Nikolics,, Anthony J. Mason, va Sznyi, J. Ramachandran& Peter H. Seeburg, A prolactin-inhibiting factor within the precursor for human gonadotropin-releasing hormone, Nature316, 511-517 (8 August 1985) doi:10.1038/316511a0
2.  WhiteSA, KastenTL, BondCT, AdelmanJP, FernaldRD.Three gonadotropin-releasing hormone genes in one organism suggest novel roles for an ancient peptide.Proc Natl Acad SciUSA92:8363–8367,1995.
3.  Tianbao Song,Karoly Nikolics, Peter H. Seeburg, Paul C. Goldsmith, GnRH-prohormone-containing neurons in the primate brain: Immunostaining for the GnRH-Associated peptide, Peptides, Volume 8, Issue 2, March–April 1987, Pages 335–346
4.  RonchiE.·AokiC.·KreyL.C.·PfaffD.W.
Immunocytochemical Study of GnRH and GnRH-Associated Peptide in Male Syrian Hamsters as a Function of Photoperiod and Gonadal Alterations, Neuroendocrinology 1992;55:134–145(DOI:10.1159/000126108)
5.  I.J. Clarke, J.T. Cummins, F.J. Karsch, P.H. Seeburg, K. Nikolics,GnRH-associated peptide (GAP) is cosecreted with GnRH into the hypophyseal portal blood of ovariectomized sheep, Biochemical and Biophysical Research Communications, Volume 143, Issue 2, 13 March 1987, Pages 665–671