CID-1067700
Ras相关GTP酶核苷酸结合的竞争性抑制剂
| 规格 | 价格 | 货期 | 数量 |
|---|---|---|---|
| 5mg | 请咨询 | 7-15工作日发货 | |
| 10mg | 请咨询 | 7-15工作日发货 | |
| 25mg | 请咨询 | 7-15工作日发货 |
特色产品
- 适用于流式细胞仪和荧光显微镜检测
- 准确区分活细胞/凋亡细胞/坏死细胞
- 用于免疫印迹和质谱分析等后续操作
- 适用于30 KDa-130 KDa大小的蛋白
- 对细胞无毒性
- 步骤更简单，无需有机溶剂
- 可将信号灵敏度提高100倍
- 同时保持稳定的特异性和分辨率
- 提供更高的转录效率并抑制免疫激活
- 使用5-moUTP和Cy5-utp修饰
- 优异的扩增效率、可重复性和稳定性
- 卓越的灵敏度和准确性，操作简单
- 优化的缓冲液和T7 RNA聚合酶混合物
- 20 μL反应体系可获得更高的RNA产量
产品描述
CID-1067700 is the first competitive inhibitor of nucleotide binding by Ras-related GTPases [1].
The Ras superfamily of GTPases, which includes Arf, Rho, Ras and Rab GTPase subfamilies, regulate many cellular processes ranging from membrane trafficking to the control of cell proliferation. Alteration of small GTPase functions is a hallmark of genetic and sporadic human diseases, making GTPase family members attractive targets [1].
CID-1067700 is a competitive inhibitor of nucleotide binding by Ras-related GTPases. CID-1067700 exhibited EC50 values of 20-500 nM and at least 40% inhibitory activity against all tested GTPases. CID-1067700 significantly inhibited Rab GTPases (Rab2 and Rab7). CID 1067700 inhibited Rab7 binding of the BODIPY-linked nucleotides with EC50 values and Ki values of 11.22 ± 1.34 nM and 12.89 nM for BODIPY-GTP, and 20.96 ± 1.34 nM and 19.70 nM for BODIPY-GDP, respectively. The maximal inhibitory response was ≥97% for both nucleotides [1].
Reference:
[1]. Agola JO, Hong L, Surviladze Z, et al. A competitive nucleotide binding inhibitor: in vitro characterization of Rab7 GTPase inhibition. ACS Chem Biol. 2012 Jun 15;7(6):1095-108.
产品性质
| 物理外观 | A crystalline solid |
| CAS号 | 314042-01-8 |
| 分子式 | C18H18N2O4S2 |
| 分子量 | 390.5 |
| 化学名称 | 2-[[(benzoylamino)thioxomethyl]amino]-4,7-dihydro-5,5-dimethyl-5H-thieno[2,3-c]pyran-3-carboxylic acid |
| 溶解度 | ≤10mg/ml in DMSO |
| SMILES | CC(C)(C1)OCc2c1c(C(O)=O)c(NC(NC(c1ccccc1)=O)=S)[s]2 |
| 存储条件 | -20°C |
| 运输条件 | 蓝冰 |
产品应用 (实验数据来自文献，APExBIO并未验证，仅供参考)
IC50和靶点
| 生物活性描述 | CID-1067700 (ML282) 是一种泛 GTPase 抑制剂；对脑内 Ras 相关 7（Rab7）具有竞争性抑制作用；Ki 为 13 nM。 |
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