Chlorothricin
| 规格 | 价格 | 货期 | 数量 |
|---|---|---|---|
| 1mg | ￥3366.00 | 10-15工作日发货 | |
| 5mg | ￥13332.00 | 10-15工作日发货 |
特色产品
- 适用于流式细胞仪和荧光显微镜检测
- 准确区分活细胞/凋亡细胞/坏死细胞
- 用于免疫印迹和质谱分析等后续操作
- 适用于30 KDa-130 KDa大小的蛋白
- 对细胞无毒性
- 步骤更简单，无需有机溶剂
- 可将信号灵敏度提高100倍
- 同时保持稳定的特异性和分辨率
- 提供更高的转录效率并抑制免疫激活
- 使用5-moUTP和Cy5-utp修饰
- 优异的扩增效率、可重复性和稳定性
- 卓越的灵敏度和准确性，操作简单
- 优化的缓冲液和T7 RNA聚合酶混合物
- 20 μL反应体系可获得更高的RNA产量
产品描述
IC50: 173, 500, 260, and 120 μM for pyruvate carboxylases from Bacillus, Azotobacter, rat, and chicken, respectively.
Chlorothricin is a macrolide-type antibiotic.
Macrolides, a class of natural products belonging to the polyketide class of natural products, consist of a large macrocyclic lactone ring. The lactone rings are oftem 14-, 15-, or 16-membered. Some macrolides have been reported to have antibiotic or antifungal activity and are widely used as pharmaceutical drugs.
In vitro: In a previous study, chlorothricin was found to inhibit the reaction catalyzed by pyruvate carboxylase from Bacillus stearothermophilus. Moreover, with steady-state kinetic measurements, inhibition of the overall reaction was found to be competitive with the allosteric activator of this enzyme, CoASAc, and non-competitive with respect to both substrates of MgATP and pyruvate. These findings strongly indicated that the site 1conformation of pyruvate carboxylase responsible for the regulation of the overall enzyme activity could be influenced by chlorothricin and CoASAc in an antagonistic manner [1].
In vivo: Up to now, there is no animal in vivo data reported.
Clinical trial: So far, no clinical study has been conducted.
Reference:
[1] Schindler PW, Zhner H. Mode of action of the macrolide-type antibiotic, chlorothricin. Kinetic study of the inhibition of pyruvate carboxylase from Bacillus stearothermophilus. Eur J Biochem. 1973 Nov 15;39(2):591-600.
产品性质
| 物理外观 | A white solid |
| CAS号 | 34707-92-1 |
| 分子式 | C50H63ClO16 |
| 分子量 | 955.5 |
| 化学名称 | (4S,4aS,6aR,11E,12aR,15R,16aS,21aR,21bR)-4-[[4-O-[3-O-(3-chloro-6-methoxy-2-methylbenzoyl)-2,6-dideoxy-β-D-arabino-hexopyranosyl]-2,6-dideoxy-β-D-arabino-hexopyranosyl]oxy]-1,2,3,4,4a,6a,7,8,9,10,12a,15,16,21,21a,21b-hexadecahydro-22-hydroxy-15,21a-dimeth |
| 溶解度 | Soluble in ethanol;Soluble in methanol;Soluble in DMSO;Soluble in dimethyl formamide |
| SMILES | OC([C@]1([C@@]([H])(C=C([C@@H](C1)C)C(O)=O)/C=C\CCCC[C@]2([H])C=C[C@@]3([H])[C@](CCC[C@@H]3O[C@@]([H])(C[C@H]4O)O[C@@H]([C@H]4O[C@@]([H])(C[C@H]5OC(C6=C(OC)C=CC(Cl)=C6C)=O)O[C@@H]([C@H]5O)C)C)([H])[C@@]27C)OC8=O)=C8OC7=O |
| 存储条件 | -20°C |
| 运输条件 | 蓝冰 |
产品应用 (实验数据来自文献，APExBIO并未验证，仅供参考)
IC50和靶点
| 生物活性描述 | Chlorothricin 是猪心苹果酸脱氢酶的抑制剂。 |
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