Chelerythrine Chloride
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Chelerythrine Chloride是PKC的有效选择性拮抗剂，IC50值为0.66 μM[1]。
生物碱chelerythrine是作用于激酶调节结构域的高特异性抑制剂[2]。相对于磷酸盐受体，它是一个竞争性抑制剂，对于ATP来说，它是非竞争性抑制剂[1]。Chelerythrine以剂量依赖的方式减少细胞活力，通过MTT法测得IC50值为2.6 μM[3]。Chelerythrine是Bcl-xL的强效选择性抑制剂，可以诱导MEF细胞死亡，IC50值为1.1 μM[4]。Chelerythrine激活MEKK1和MKK4依赖性的JNK1和p38途径，诱导细胞凋亡[5]。在大鼠体内实验中，5 mg/kg 的chelerythrine通过诱导活性氧的产生刺激细胞凋亡[6]。Chelerythrine对抗微生物和抗炎过程也具有广泛的生理作用。
参考文献：
1. J. M. Herbert, J. M. Augereau, J. Gleye and J. P. Maffrand, Biochem Biophys Res Commun 1990, 172, 993-999.
2. W. D. Jarvis, A. J. Turner, L. F. Povirk, R. S. Traylor and S. Grant, Cancer Res 1994, 54, 1707-1714.
3. J. Vrba, P. Dolezel, J. Vicar, M. Modriansky and J. Ulrichova, Toxicol In Vitro 2008, 22, 1008-1017.
4. M. Vogler, K. Weber, D. Dinsdale, I. Schmitz, K. Schulze-Osthoff, M. J. Dyer and G. M. Cohen, Cell Death Differ 2009, 16, 1030-1039.
5. R. Yu, S. Mandlekar, T. H. Tan and A. N. Kong, J Biol Chem 2000, 275, 9612-9619.
6. S. Yamamoto, K. Seta, C. Morisco, S. F. Vatner and J. Sadoshima, J Mol Cell Cardiol 2001, 33, 1829-1848.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 383.83 |
| Cas No. | 3895-92-9 |
| Formula | C21H18ClNO4 |
| Solubility | insoluble in H2O; ≥19.2 mg/mL in DMSO; ≥9.45 mg/mL in EtOH with gentle warming and ultrasonic |
| Chemical Name | 1,2-dimethoxy-12-methyl-[1,3]benzodioxolo[5,6-c]phenanthridin-12-ium |
| SDF | Download SDF |
| Canonical SMILES | C[N+]1=C2C(=C3C=CC(=C(C3=C1)OC)OC)C=CC4=CC5=C(C=C42)OCO5.[Cl-] |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
