Brefeldin A
| 规格 | 价格 | 货期 | 数量 |
|---|---|---|---|
| 1mL(10 mM in DMSO) | ￥545.00 | 现货 | |
| 5mg | ￥454.00 | 现货 | |
| 25mg | ￥1727.00 | 现货 | |
| 100mg | ￥4272.00 | 现货 |
特色产品
- 适用于流式细胞仪和荧光显微镜检测
- 准确区分活细胞/凋亡细胞/坏死细胞
- 用于免疫印迹和质谱分析等后续操作
- 适用于30 KDa-130 KDa大小的蛋白
- 对细胞无毒性
- 步骤更简单，无需有机溶剂
- 可将信号灵敏度提高100倍
- 同时保持稳定的特异性和分辨率
- 提供更高的转录效率并抑制免疫激活
- 使用5-moUTP和Cy5-utp修饰
- 优异的扩增效率、可重复性和稳定性
- 卓越的灵敏度和准确性，操作简单
- 优化的缓冲液和T7 RNA聚合酶混合物
- 20 μL反应体系可获得更高的RNA产量
产品描述
Brefeldin A（布雷菲德菌素A，BFA）是ATP酶的抑制剂，IC50值为0.2 μM[1]。
ATP酶是一种化学酶，在ADP/ATP交换中是必不可少的，而ADP/ATP交换过程提供化学势能。ATP为许多生理活动提供能量，如为细胞代谢输入必需的代谢产物，输出阻碍细胞过程的毒素、废物及溶质，细胞增殖，ER应激等等[2]。
BFA通过抑制小泡胞吐，从而减弱刺激依赖性的痛觉过敏现象。而小泡胞吐过程对ATP的释放是重要的[3]。在稳定表达野生型CRELD2的HEK293细胞系中，BFA通过抑制蛋白从ER向高尔基体的运输，几乎完全废除wtCRELD2的分泌[4]。在MCF-7细胞和Hela细胞中，BFA通过抑制ATP，增强ER应激，从而诱导p53的表达[5]。在大肠癌细胞系HCT116细胞中，BFA通过抑制ATP，诱导细胞凋亡，而ATP在细胞囊泡运输中起作用[1]。
据报道，BFA也以剂量依赖的方式抑制GTP/GDP交换，其对囊泡运输也是重要的[6]。
参考文献：
[1] P. M. Wierzbicki, M. Kogut, J. Ruczynski, K. Siedlecka-Kroplewska, L. Kaszubowska, A. Rybarczyk, M. Alenowicz, P. Rekowski, Z. Kmiec, Protein and siRNA delivery by transportan and transportan 10 into colorectal cancer cell lines, Folia Histochem Cytobiol, (2014).
[2] M. Westerterp, A.E. Bochem, L. Yvan-Charvet, A.J. Murphy, N. Wang, A.R. Tall, ATP-binding cassette transporters, atherosclerosis, and inflammation, Circulation research, 114 (2014) 157-170.
[3] E. K. Joseph, P.G. Green, J.D. Levine, ATP release mechanisms of endothelial cell-mediated stimulus-dependent hyperalgesia, The journal of pain : official journal of the American Pain Society, 15 (2014) 771-777.
[4] K. Oh-hashi, Y. Kanamori, Y. Hirata, K. Kiuchi, Characterization of V-ATPase inhibitor-induced secretion of cysteine-rich with EGF-like domains 2, Cell biology and toxicology, 30 (2014) 127-136.
[5] W. C. Lin, Y.C. Chuang, Y.S. Chang, M.D. Lai, Y.N. Teng, I.J. Su, C.C. Wang, K.H. Lee, J.H. Hung, Endoplasmic reticulum stress stimulates p53 expression through NF-kappaB activation, PLoS One, 7 (2012) e39120.
[6] D. Prieto, P. Corchete, Transport of flavonolignans to the culture medium of elicited cell suspensions of Silybum marianum, Journal of plant physiology, 171 (2014) 63-68.
产品性质
| 物理外观 | A solid |
| CAS号 | 20350-15-6 |
| 分子式 | C16H24O4 |
| 分子量 | 280.36 |
| 化学名称 | (1R,2E,6S,10E,11aS,13S,14aR)-1,13-dihydroxy-6-methyl-6,7,8,9,12,13,14,14a-octahydro-1H-cyclopenta[f][1]oxacyclotridecin-4(11aH)-one |
| 溶解度 | insoluble in H2O; ≥11.73 mg/mL in EtOH with ultrasonic; ≥4.67 mg/mL in DMSO |
| SMILES | C[C@@H](CCC/C=C/[C@H](C[C@@H](C1)O)[C@@H]1[C@@H](/C=C1)O)OC\1=O |
| 存储条件 | -20°C |
| 运输条件 | 蓝冰 |
产品应用 (实验数据来自文献，APExBIO并未验证，仅供参考)
IC50和靶点
| 生物活性描述 | 布雷非德菌素 A（BFA）是一种内酯类抗生素，也是蛋白质运输的特异性抑制剂。Brefeldin A 能阻止分泌蛋白和膜蛋白从内质网向高尔基体的转运。Brefeldin A 还是一种自噬和有丝分裂抑制剂。Brefeldin A 是一种 CRISPR/Cas9 激活剂。Brefeldin A 可抑制 HSV-1，并具有抗癌活性。 |
生物相关数据
APExBIO 顾客使用本产品发表的 13 篇科研文献
- 1. Emily R. Derbyshire, et al. "Toxoplasma and Plasmodium associate with host Arfs during infection." mSphere. 2024 Mar 26;9(3):e0077023. PMID: 38349168
- 2. Ly Thi Huong Luu Le, Seoyoung Park, et al. "N-recognins UBR1 and UBR2 as central ER stress sensors in mammals." Molecules and Cells. January 2024.
- 3. Jing-Ya Yang, Fang Wang, et al. "Characterization and function of circulating mucosal‐associated invariant T cells and γδT cells in oral lichen planus." J Oral Pathol Med. 2021 Oct 12. PMID: 34637577
- 4. KE PENG, AIQIN SUN, et al. "Restoration of the ATG5‑dependent autophagy sensitizes DU145 prostate cancer cells to chemotherapeutic drugs." Oncol Lett. 2021 Sep;22(3):638. PMID: 34386060
- 5. Jun Wang, Wei Tang, et al. "Inflammatory tumor microenvironment responsive neutrophil exosomes-based drug delivery system for targeted glioma therapy." Biomaterials. 2021 Jun;273:120784. PMID: 33848731
- 6. Yikun Chen, Jiajia Wang, et al. "Moesin Is a Novel Biomarker of Endothelial Injury in Sepsis." J Immunol Res. 2021 Feb 13;2021:6695679. PMID: 33628853
- 7. Yulong Fu, Yang Zhang, et al. "Abnormally activated OPN/integrin αVβ3/FAK signalling is responsible for EGFR-TKI resistance in EGFR mutant non-small-cell lung cancer." J Hematol Oncol. 2020 Dec 7;13(1):169. PMID: 33287873
- 8. Wenwen Du, Jianjie Zhu, et al. "KPNB1-mediated nuclear translocation of PD-L1 promotes non-small cell lung cancer cell proliferation via the Gas6/MerTK signaling pathway." Cell Death Differ. 2021 Apr;28(4):1284-1300. PMID: 33139930
- 9. Raphemot R, Toro-Moreno M, et al. "Discovery of Druggable Host Factors Critical to Plasmodium Liver-Stage Infection." Cell Chem Biol. 2019 Jun 12. pii: S2451-9456(19)30180-1. PMID: 31257182
- 10. Brian D. Rutter. "CONTENTS AND FUNCTIONS OF EXTRACELLULAR VESICLES ISOLATED FROM PLANTS." Indiana University. 2019.
- 11. Zhang M, Sun H, et al. "COPI-Mediated Nuclear Translocation of EGFRvIII Promotes STAT3 Phosphorylation and PKM2 Nuclear Localization." Int J Biol Sci. 2019 Jan 1;15(1):114-126. PMID: 30662352
- 12. Liu C, Zhang Y, Ren H. "Actin Polymerization Mediated by AtFH5 Directs the Polarity Establishment and Vesicle Trafficking for Pollen Germination in Arabidopsis." Mol Plant. 2018 Nov 5;11(11):1389-1399. PMID: 30296598
- 13. Rutter BD, Innes RW. "Extracellular Vesicles Isolated from the Leaf Apoplast Carry Stress-Response Proteins." Plant Physiol. 2017 Jan;173(1):728-741. doi:10.1104/pp.16.01253. PMID: 27837092
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