Blasticidin S HCl
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Blasticidin S HCl是一种抑制蛋白合成的抗生素 [1].
Blasticidin S分离于Streptomyces griseochromogenes,可抑制原核和真核细胞的蛋白质合成.
Blasticidin S HCl是一种抑制蛋白质合成的抗生素.在小鼠白血病L5178Y细胞中,blasticidin S可抑制蛋白质合成,并可导致推定mRNA的降解 [1].并且,blasticidin S可以剂量依赖的方式抑制Dictyostelium discoideum的核糖核酸酶P活性,并可抑制tRNA成熟 [2].在S. cerevisiae中,blasticidin S可抑制半乳糖诱导的蛋白质合成.在Aspergillus parasiticus中,blasticidin S可抑制黄曲霉素的产生,而不抑制真菌生长 [3].在Aspergillus flavus中,blasticidin S显著减少黄曲霉素合成酶 [3].Blasticidin S可有效结合Cu2+离子,保护DNA免遭金属诱发的损伤 [4].Blasticidin S可提高tRNA结合核糖体大亚基的P位点,减缓自发的亚基间轮换.Blasticidin S可抑制肽键形成和肽基-tRNA水解 [5].
参考文献：
[1]. Takenaka K, Endo H, Kuwano M. The synthesis and degradation of presumptive messenger RNA in cultured mouse leukemia cells during the inhibition of protein synthesis. Biochim Biophys Acta, 1978, 521(1): 295-307.
[2]. Stathopoulos C, Tsagla A, Tekos A, et al. Effect of peptidyltransferase inhibitors on ribonuclease P activity from Dictyostelium discoideum. Effect of antibiotics on RNase P. Mol Biol Rep, 2000, 27(2): 107-111.
[3]. Yoshinari T, Noda Y, Yoda K, et al. Inhibitory activity of blasticidin A, a strong aflatoxin production inhibitor, on protein synthesis of yeast: selective inhibition of aflatoxin production by protein synthesis inhibitors. J Antibiot (Tokyo), 2010, 63(6): 309-314.
[4]. Stokowa-Sotys K, Jeowska-Bojczuk M. A rice fungicide blasticidin S efficiently binds Cu(II) ions and prevents DNA from metal-induced damage. J Inorg Biochem, 2013, 127: 73-78.
[5]. Svidritskiy E, Ling C, Ermolenko DN, et al. Blasticidin S inhibits translation by trapping deformed tRNA on the ribosome. Proc Natl Acad Sci U S A, 2013, 110(30): 12283-12288.
| Physical Appearance | White solid |
| Storage | Store at -20°C |
| M.Wt | 458.9 |
| Cas No. | 3513-03-9 |
| Formula | C17H27ClN8O5 |
| Solubility | insoluble in DMSO; insoluble in EtOH; ≥52.4 mg/mL in H2O |
| Chemical Name | (2S,3S,6R)-3-[[(3R)-3-amino-5-[carbamimidoyl(methyl)amino]pentanoyl]amino]-6-(4-amino-2-oxopyrimidin-1-yl)-3,6-dihydro-2H-pyran-2-carboxylic acid;hydrochloride |
| SDF | Download SDF |
| Canonical SMILES | CN(CCC(CC(=O)NC1C=CC(OC1C(=O)O)N2C=CC(=NC2=O)N)N)C(=N)N.Cl |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Cell experiment:[1] | |
|
Cell lines |
Mouse leukemia L5178Y cells |
|
Reaction Conditions |
20 μg/ml blasticidin S for 6 h incubation |
|
Applications |
In mouse leukemia L5178Y cells, blasticidin S resulted in a marked accumulation of monosome and ribosomal subunits, accompanied by a drastic decrease of pulse-labeled RNA (presumably mRNA) in the larger size region of polysomes. In response to a decreased protein synthesis (5 ~ 10% of the control), the appearance of pulse-labeled [3H]RNA among polysomes and monosome decreased to approximately 10% of the control when cells were treated with blasticidin S. |
|
Note |
The technical data provided above is for reference only. |
|
References: 1. Takenaka K, Endo H, Kuwano M. The synthesis and degradation of presumptive messenger RNA in cultured mouse leukemia cells during the inhibition of protein synthesis. Biochimica et Biophysica Acta, 1978, 521 (1): 295-307. |
|
化学结构
