AM580
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
AM580是视黄酸受体α(RARα)的选择性激动剂,IC50值和EC50值分别为8 nM和0.36 nM [1].
RARα普遍表达,其在上皮细胞的生长和分化期间对维甲酸响应发挥重要作用.RARα可以抑制细胞和乳腺瘤生长,延长肿瘤潜伏期,诱导细胞周期停滞,刺激细胞死亡和上调RARα及RARγ的mRNA[1, 2].另外,RARα参与人T细胞活化和2型细胞因子产生[3].
在SK-BR-3和T47D乳腺癌细胞系中,1 nM和10 nM的AM580处理7天能降低细胞数量[1].在RAR-γ 敲减的MCF-10A乳腺上皮细胞和MCF-7细胞系中,200 nM的AM580能增强抗肿瘤效应[2].
200 nM AM580处理MMTV-Myc雌性小鼠96小时能够损伤细胞增殖能力.而且,AM580与100 nM CD437和SR11253联合给药能导致强烈的生长抑制,表明MMTV-Myc肿瘤模型中AM580抑制肿瘤生长的能力[2].
参考文献：
[1]. Schneider S, Offterdinger M, Huber H, et al. Activation of Retinoic Acid Receptor a Is Sufficient for Full Induction of Retinoid Responses in SK-BR-3 and T47D Human Breast Cancer Cells. Cancer Res, 2000, 60(19):5479-87.
[2]. Bosch A, Bertran S, Lu Y, et al. Reversal by RARa agonist Am580 of c-Myc induced imbalance in RARa/RARγ expression during MMTV-Myctumorigenesis. Breast Cancer Res, 2012, 14(4):R121.
[3]. Dawson H, Collins G, Pyle R, et al. The Retinoic Acid Receptor-α mediates human T-cell activation and Th2 cytokine and chemokine production. BMC Immunol, 2008, 9:16.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 351.44 |
| Cas No. | 102121-60-8 |
| Formula | C22H25NO3 |
| Solubility | insoluble in H2O; ≥14.3 mg/mL in DMSO; ≥18.37 mg/mL in EtOH with ultrasonic |
| SDF | Download SDF |
| Canonical SMILES | O=C(C1=CC=C2C(C)(C)CCC(C)(C)C2=C1)NC3=CC=C(C(O)=O)C=C3 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
