5,7-dihydroxychromone
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
5,7-Dihydroxychromone是从植物中(如花生壳)提取的天然抗氧化剂.5,7-Dihydroxychromone是Nrf2 / ARE信号激活剂,ROS抑制剂[1];及PPARγ激动剂[2].
NF-E2相关因子2(Nrf2)/抗氧化反应元件(ARE)信号对抗氧化基因表达的诱导有重要作用,Nrf2的活化可以中和氧化应激.在正常状态下,Nrf2位于细胞质中,抗氧化剂可诱导Nrf2的核转位,随后使Nrf2 / ARE复合物介导抗氧化酶基因的诱导.
过氧化物酶体增殖物激活受体(PPARs)是核受体家族的成员;PPAR同种型(α,β/δ和γ)是葡萄糖吸收,脂质代谢,增殖和细胞分化的关键调节剂.PPARγ激动剂通常作为胰岛素敏化剂,用于治疗2型糖尿病.
体外:用5,7-Dihydroxychromone(DHC)(0.4-10 μM)处理6-OHDA诱导的SH-SY5Y细胞,发现DHC可保护神经元细胞免于死亡并抑制ROS的产生,具有量效关系.DHC(0.08-10 μM)可剂量依赖性地增加核Nrf2的诱导,其与ARE结合并激活ARE驱动的II期抗氧化酶,NQO1,HO-1和GCLc [1].交让木果实提取物(DME,体内实验)及其主要成分5,7-Dihydroxychromone (1,5和10 μg/ mL,体外实验)的处理以剂量依赖方式增加3T3-L1细胞中PPARγ和LXRα的mRNA表达,5,7-Dihydroxychromone(1 μg/mL)也有效增加脂肪细胞的分化,表明其作为PPARγ激动剂起作用,具有抗糖尿病特性[2].5,7-Dihydroxychromone可抑制H9淋巴细胞中HIV的复制 [3].
References:
[1]. Kim D W, Lee K, Kwon J, et al. Neuroprotection against 6-OHDA-induced oxidative stress and apoptosis in SH-SY5Y cells by 5, 7-Dihydroxychromone: Activation of the Nrf2/ARE pathway[J]. Life sciences, 2015, 130: 25-30.
[2]. Koo H J, Kwak J H, Kang S C. Anti-diabetic properties of Daphniphyllum macropodum fruit and its active compound[J]. Bioscience, biotechnology, and biochemistry, 2014, 78(8): 1392-1401.
[3]. Wu P L, Lin F W, Wu T S, et al. Cytotoxic and anti-HIV principles from the rhizomes of Begonia nantoensis[J]. Chemical and pharmaceutical bulletin, 2004, 52(3): 345-349.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 178.14 |
Cas No. | 31721-94-5 |
Formula | C9H6O4 |
Solubility | insoluble in H2O; ≥5.41 mg/mL in EtOH with gentle warming; ≥7.05 mg/mL in DMSO |
Chemical Name | 5,7-dihydroxychromen-4-one |
SDF | Download SDF |
Canonical SMILES | C1=COC2=CC(=CC(=C2C1=O)O)O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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