3-Deazaadenosine
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Ki为3.9 μM。
3-Deazaadenosine是S-腺苷高半胱氨酸（SAH）水解酶的抑制剂。
S-腺苷高半胱氨酸（SAH）水解酶可以催化SAH可逆水解形成腺苷和同型半胱氨酸。SAH水解酶的抑制导致SAH的细胞内积累，提高SAH与S-腺苷甲硫氨酸（SAM）的比例，并抑制SAM依赖性甲基转移酶。
体外：3-Deazaadenosine在灵长类的多种细胞系（SW13、Vero 76、FRhL、LLC-MK2、MRC-5、Vero E6）和小鼠细胞系（BALB/3T3克隆A31）中表现出对EBO-Z病毒、EBO和Marburg病毒的抑制值。2 μg/mL 的3-Deazaadenosine可以剂量依赖性地减少3 logs的病毒复制。然而，即使浓度增加100倍也没有进一步的抑制[1]。
体内：在载体对照组中，成年BALB/c小鼠在致死性感染小鼠适应性Ebola病毒5-7天后死亡。相比之下，在第0天或1天开始3-Deazaadenosine治疗导致剂量依赖性保护，在每8小时约0.7 mg/kg的剂量下完全阻止死亡。此外，当在第2天开始3-Deazaadenosine治疗时，具有显著的保护，此时，脾的平均滴度为2×10 6 pfu/g，肝脏具有3×10 5 pfu/g病毒。在第3天开始用2.2 mg / kg的3-aeazaadenosine治疗导致40％的存活率 [1]。
临床试验：到目前为止，3-Deazaadenosine仍处于临床前研究阶段。
参考文献：
[1] Huggins, Z. X. Zhang and M. Bray.Antiretroviral drug therapy of filovirus infections: S-adenosylhomocysteine hydrolase inhibitors inhibit ebola virusin vitroand in a lethal mouse model.Journal of Infectious Diseases179(1),S240-S247(1999).
- 1. Hongli Jiao, Lijie Chen, et al. "IGF2BP3 promotes progression of colorectal cancer and mediates cetuximab resistance by stabilizing EGFR mRNA in an m6A-dependent mechanism." Research Square. rs-1297958/v1.
- 2. Hao Wang, Wei Wei, et al. "TCF4 and HuR Mediated-METTL14 Suppresses Dissemination of Colorectal Cancer via N6-Methyladenosine-Dependent Silencing of ARRDC4." Cell Death Dis. 2021 Dec 17;13(1):3. PMID:34916487
- 3. Chen Y, Peng C, et al. "WTAP facilitates progression of hepatocellular carcinoma via m6A-HuR-dependent epigenetic silencing of ETS1." Mol Cancer. 2019 Aug 22;18(1):127. PMID:31438961
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 266.25 |
| Cas No. | 6736-58-9 |
| Formula | C11H14N4O4 |
| Solubility | ≥26.6 mg/mL in DMSO; insoluble in EtOH; ≥7.53 mg/mL in H2O with gentle warming |
| Chemical Name | (2R,3R,4S,5R)-2-(4-amino-1H-imidazo[4,5-c]pyridin-1-yl)-5-(hydroxymethyl)tetrahydrofuran-3,4-diol |
| SDF | Download SDF |
| Canonical SMILES | NC1=C2C(N(C=N2)[C@H]3[C@@H]([C@@H]([C@H](O3)CO)O)O)=CC=N1 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
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化学结构
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