24(R)-hydroxy Cholesterol
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
EC50: 7 and 4 μM for LXRα and LXRβ, respectively
24(R)-hydroxy Cholesterol is a LXRα and LXRβ nuclear receptors activator.
The liver X receptors (LXRs) are identified as orphan members of the nuclear receptor superfamily. Like other receptors in the family, LXRs heterodimerize with RXR and bind to specific response elements. LXRα and β are nuclear receptors regulating the metabolism of several important lipids, such as cholesterol and bile acids.
In vitro: In previous study, the authors proposed that LXRs could regulate the lipid metabolism via their interaction with specific oxysterols, such as 24(R)-hydroxycholesterol, 22(R)-hydroxycholesterol, 24(S)-hydroxycholesterol, and 24(S),25-epoxycholesterol. Results showed that by using a ligand binding assay, it was demonstrated that these oxysterols could directly bind to LXRs. In addition, this study further revealed that the position-specific monooxidation of the sterol side chain was required for LXR binding and activation. Enhanced binding and activation could be achieved via the use of 24-oxo ligands. Moreover, the introduction of an oxygen on the sterol B-ring led to a LXRa-subtype selectivity [1].
In vivo: Up to now, there is no animal in vivo data reported.
Clinical trial: So far, no clinical study has been conducted.
Reference:
[1] Janowski, B. A.,Grogan, M.J.,Jones, S.A., et al. Structural requirements of ligands for the oxysterol liver X receptors LXRα and LXRβ. Proceedings of the National Academy of Sciences of the United States of America 96(1), 266-271 (1999).
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 402.7 |
Cas No. | 27460-26-0 |
Formula | C27H46O2 |
Synonyms | 24-Epicerebrosterol |
Solubility | ≤20mg/ml in ethanol;0.1mg/ml in DMSO;2mg/ml in dimethyl formamide |
Chemical Name | cholest-5-ene-3β,24R-diol |
SDF | Download SDF |
Canonical SMILES | O[C@H](C1)CC[C@@]2(C)C1=CC[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@]4([H])[C@H](C)CC[C@@H](O)C(C)C |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |