Z-VEID-FMK
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
与其它的caspase抑制剂相比，Z-DRHD-FMK比一般的caspase抑制剂Z-Val-Ala-Asp (OMe)-氟甲基酮（Z-VAD-FMK）或caspase 6抑制剂Z-Val-Glu(Ome)-Ile-Asp(OMe)-氟甲基酮（Z-VEID-FMK）更加有效地抑制caspase 6的活性。然而，Z-DRHD-FMK对TNFα诱导的细胞凋亡的抑制作用不如Z-VAD-FMK或Z-VEID-FMK有效，大概是因为caspase 6只是至少三个效应caspases之一，其它两个是caspase 3和7，而它们在caspase依赖的细胞凋亡中也是活跃的。caspase-6偏爱的抑制剂（Z-VEID-FMK）可以阻止DNA结合活性的丧失和TNFα诱导的细胞凋亡[1]。
Caspase-6特异性的抑制剂Z-VEID-FMK和一般的caspase抑制剂可显著阻止caspase-6显微注射神经元的凋亡[2]。
Z-VEID-FMK抑制caspase-6，也能够废除procaspase-8的裂解，尽管在Fas介导的细胞凋亡中，caspase-6位于caspase-8的下游，被caspase-8所激活[3]。
参考文献：
1. Nyormoiet al(2003) Sequence-based discovery of a synthetic peptide inhibitor of caspase 6. Apoptosis8371.
2. Y. Zhang C. Goodyer, Selective and Protracted Apoptosis in Human Primary Neurons Microinjected with Active Caspase-3, -6, -7, and -8. The Journal of Neuroscience, 2000, 20(22):8384–8389
3. Microinjected with Active Caspase-3, -6, -7, and -8 Taimen and Kallajoki(2003) NuMA and nuclear lamins behave differently in Fas-mediated apoptosis. J.Cell Sci.116571.
- 1. Hin Chu, Yuxin Hou, et al. "Coronaviruses exploit a host cysteine-aspartic protease for replication." Nature. 2022 Aug 3. PMID: 35922005
- 2. Hodges AL, Kempen CG, et al. "TNF Family Cytokines Induce Distinct Cell Death Modalities in the A549 Human Lung Epithelial Cell Line when Administered in Combination with Ricin Toxin." Toxins (Basel). 2019 Aug 1;11(8). pii: E450. PMID: 31374990
- 3. Xie J, Guo H, et al. "Identification of cleavage of NS5A of C-strain classical swine fever virus." Arch Virol. 2016 Oct 20. PMID: 27766426
- 4. Chen Y, Sun M, et al, "SM-1, a novel PAC-1 derivative, activates procaspase-3 and causes cancer cell apoptosis." Cancer Chemother Pharmacol. 2016 Aug 3. PMID: 27488460
| Storage | Store at -20°C |
| M.Wt | 652.71 |
| Formula | C31H45FN4O10 |
| Synonyms | Caspase-6 inhibitor (fluoromethylketone),Benzyloxycarbonyl-Val-Glu(OMe)-Ile-Asp(OMe)-fluoromethylketone |
| Solubility | insoluble in H2O; ≥113.4 mg/mL in DMSO; ≥3.01 mg/mL in EtOH with gentle warming and ultrasonic |
| Chemical Name | methyl (4S)-5-[[(2S)-1-[[(3S)-5-fluoro-1-methoxy-1,4-dioxopentan-3-yl]amino]-3-methyl-1-oxopentan-2-yl]amino]-4-[[(2S)-3-methyl-2-(phenylmethoxycarbonylamino)butanoyl]amino]-5-oxopentanoate |
| SDF | Download SDF |
| Canonical SMILES | CCC(C)C(C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)C(CCC(=O)OC)NC(=O)C(C(C)C)NC(=O)OCC1=CC=CC=C1 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Cell experiment [1]: | |
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细胞系 |
黄体细胞 |
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制备方法 |
溶于DMSO。若配制更高浓度的溶液，一般步骤如下：请将试管置于37℃加热10分钟和/或将其置于超声波浴中震荡一段时间。原液于-20℃可放置数月。 |
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反应条件 |
50 μM；6小时 |
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实验结果 |
对于黄体细胞，与caspase-3，caspase-6和caspase-8抑制剂（分别为Z-DEVD-FMK，Z-VEID-FMK，Z-IETD-FMK）以及一般的caspase抑制剂（Boc-DFMK）一起孵育可以减少肿瘤坏死因子α诱导的DNA片段化。 |
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References: [1]. Abdo M1, Hisheh S, Dharmarajan A. Role of tumor necrosis factor-alpha and the modulating effect of the caspases in rat corpus luteum apoptosis. Biol Reprod. 2003 Apr;68(4):1241-8. |
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| Z-VEID-FMK是caspase-6/Mch2的特异性识别序列。 | ||||||
| 靶点 | caspase-6 | |||||
| IC50 | ||||||
质量控制和MSDS
- 批次:
化学结构
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