Z-DEVD-pNA
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Z-DEVD-pNA是一种caspase-3的化学显色底物，有较高的细胞通透性。这种底物的序列是基PARP poly(ADP-ribose) polymerase上被caspase-3切割的的氨基酸位点序列DEVD。 Z-DEVD-pNA 也能辨识caspases-1, -4 和 -7。Z-DEVD-pNA的N-末端被 benzyloxycarbonyl (Z) 基团给抑制，从而提高细胞的通透性。Caspase-3的活性可以由分光光度计检测的从底物DEVD-pNA上被切割的游离的pNA的数值来决定(l = 400 nm)。在细胞凋亡的裂解物中，Z-DEVD-pNA 能够用来鉴别和量化caspase-3的活性。
References:
1. Lazebnik, Y.A.,Kaufmann, S.H.,Desnoyers, S., et al. Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE. Nature 371, 346-347 (1994).
2. Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis: D.W. Nicholson, et al.; Nature 376, 37 (1995)
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 730.68 |
| Formula | C32H38N6O14 |
| Solubility | ≥28.35 mg/mL in DMSO; ≥4.31 mg/mL in EtOH with ultrasonic; ≥7.31 mg/mL in H2O |
| Chemical Name | 8-(2-carboxyethyl)-5-(carboxymethyl)-11-isopropyl-14-((4-nitrophenyl)carbamoyl)-3,6,9,12-tetraoxo-1-phenyl-2-oxa-4,7,10,13-tetraazahexadecan-16-oic acid |
| SDF | Download SDF |
| Canonical SMILES | O=C(NC(C(NC(C(NC(C(NC(CC(O)=O)C(NC1=CC=C([N+]([O-])=O)C=C1)=O)=O)C(C)C)=O)CCC(O)=O)=O)CC(O)=O)OCC2=CC=CC=C2 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
