Wiskostatin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Wiskostatin is an N-WASP inhibitor, with an IC50 value slightly less than 10 μM. Wiskostatin interacts with a cleft in the regulatory GTPase-binding domain (GBD) of N-WASP and maintains N-WASP in an inactive conformation, thereby inhibiting Cdc42 and phosphatidylinositol 4,5-bisphosphate (PIP2) mediated N-WASP activation, as well as Arp2/3 complex activation. N-WASP, highly expressed in the brain, belongs to the WASP family in which member proteins initiate the nucleation of new actin filaments through activation of the Arp2/3 complex, and thus are emerging as critical regulators of the actin cytoskeleton. By inhibiting N-WASP, wiskostatin has been shown to interfere with the formation of dendritic spines and synapses in neurons.
References:
1. Peterson JR, Bickford LC, Morgan D, et al. Chemical inhibition of N-WASP by stabilization of a native autoinhibited conformation. Nature Structural & Molecular Biology, 2004, 11(8): 747-755.
2. Wegner AM, Nebhan CA, Hu L, et al. N-wasp and the arp2/3 complex are critical regulators of actin in the development of dendritic spines and synapses. Journal of Biological Chemistry, 2008, 283(23): 15912-15920.
| Physical Appearance | A crystalline solid |
| Storage | Store at -20°C |
| M.Wt | 426.15 |
| Cas No. | 253449-04-6 |
| Formula | C17H18Br2N2O |
| Solubility | Soluble in DMSO |
| Chemical Name | (R)-1-(3,6-dibromo-9H-carbazol-9-yl)-3-(dimethylamino)propan-2-ol |
| SDF | Download SDF |
| Canonical SMILES | BrC1=CC=C(C2=C1)N(C(C2=C3)=CC=C3Br)C[C@@H](CN(C)C)O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Cell experiment:[2] | |
|
Cell lines |
Hippocampal neurons |
|
Reaction Conditions |
2 or 5 μM wiskostatin |
|
Applications |
Wiskostatin caused a dose-dependent decrease in the number of dendritic spines and excitatory synapses. However, wiskostatin, even at the highest concentration (5 μM), did not significantly affect the number of inhibitory synapses. In addition, wiskostatin did not adversely affect the health of the neurons, because they were observed to develop normally and no detectable effects on the growth of axons or dendrites were seen at any of the wiskostatin concentrations used. |
|
Note |
The technical data provided above is for reference only. |
|
References: 1. Peterson JR, Bickford LC, Morgan D, et al. Chemical inhibition of N-WASP by stabilization of a native autoinhibited conformation. Nature Structural & Molecular Biology, 2004, 11(8): 747-755. 2. Wegner AM, Nebhan CA, Hu L, et al. N-wasp and the arp2/3 complex are critical regulators of actin in the development of dendritic spines and synapses. Journal of Biological Chemistry, 2008, 283(23): 15912-15920. |
|
质量控制和MSDS
- View current batch:
-
Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
化学结构
