Vacuolin-1
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Vacuolin-1 is a potent and cell-permeable inhibitor of Ca2+-dependent lysosomal exocytosis [1].
Exocytic fusion of lysosomes triggered by plasma membrane damage is the major source of the membrane required for resealing. Lysosomal markers appear at the cell surface or are released into the medium on transient elevation of cytosolic Ca2+, including that induced by plasma membrane disruption [1].
Vacuolin-1 is a cell-permeable blocker of Ca2+-dependent lysosomal exocytosis induced by ionomycin or plasma membrane wounding. In HeLa cells, 5 or 10 μM Vacuolin-1 reduced the release of lysosomal β-hexosaminidase. Vacuolin-1 also blocked the ionomycin-induced, Ca2+-dependent cell surface appearance of the luminal epitope from the lysosomal membrane protein, Lamp-1, a marker for fusion between the limiting membranes of lysosomes and the cell surface. Thus, vacuolin-1 blocks the Ca2+-dependent fusion of lysosomes with the plasma membrane and the release of lysosomal contents. While vacuolin-1 had no effect on the fusion of enlargeosomes with the plasma membrane. Other cell structures and membrane trafficking functions were also unaffected [1].
Reference:
[1]. Cerny J, Feng Y, Yu A, et al. The small chemical vacuolin-1 inhibits Ca2+-dependent lysosomal exocytosis but not cell resealing. EMBO Rep. 2004 Sep;5(9):883-8.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 577.4 |
Cas No. | 351986-85-1 |
Formula | C26H24IN7O |
Solubility | ≥7.28 mg/mL in DMSO with ultrasonic; insoluble in EtOH; insoluble in H2O |
Chemical Name | 3-iodo-2-[4-(diphenylamino)-6-(4-morpholinyl)-1,3,5-triazin-2-yl]hydrazone, benzaldehyde |
SDF | Download SDF |
Canonical SMILES | IC1=CC(/C=N/NC2=NC(N(C3=CC=CC=C3)C4=CC=CC=C4)=NC(N5CCOCC5)=N2)=CC=C1 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
Cell experiment:[1] | |
Cell lines |
HeLa cells |
Reaction Conditions |
1, 5 or 10 μM vacuolin-1 for 1, 2 or 4 h incubation |
Applications |
In the absence of vacuolin-1, treatment of HeLa cells for 10 min with 5 μM ionomycin resulted in the expected release of 18 ~ 20% of lysosomal β-hexosaminidase. In contrast, cells that were pretreated with 5 or 10 μM vacuolin-1 for 2 h released no more β-hexosaminidase compared with cells that were not exposed to ionomycin (~ 4%). However, pretreatment of HeLa cells with 1 μM vacuolin-1 had no effect on the cell surface appearance of the enlargeosome marker in response to ionomycin. Thus, the inhibitory effect of vacuolin-1 on Ca2+-dependent exocytosis was not a general phenomenon. Rather, it seemed to be a specific effect for endosomes and lysosomes but not for enlargeosomes. |
Note |
The technical data provided above is for reference only. |
References: 1. Cerny J, Feng Y, Yu A, et al. The small chemical vacuolin-1 inhibits Ca(2+)-dependent lysosomal exocytosis but not cell resealing. EMBO Reports, 2004, 5(9): 883-888. |
质量控制和MSDS
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