切换导航
关闭
  • 菜单
  • Setting

Vacuolin-1

 
Catalog No.
C4084
溶酶体胞吐作用抑制剂
组合的产品项目
规格价格库存 数量
5mg
¥ 1,895.00
现货
10mg
¥ 2,762.00
现货

电话: 021-55669583

邮箱: sales@apexbio.cn

全球经销商

A

背景

Vacuolin-1 is a potent and cell-permeable inhibitor of Ca2+-dependent lysosomal exocytosis [1].

Exocytic fusion of lysosomes triggered by plasma membrane damage is the major source of the membrane required for resealing. Lysosomal markers appear at the cell surface or are released into the medium on transient elevation of cytosolic Ca2+, including that induced by plasma membrane disruption [1].

Vacuolin-1 is a cell-permeable blocker of Ca2+-dependent lysosomal exocytosis induced by ionomycin or plasma membrane wounding. In HeLa cells, 5 or 10 μM Vacuolin-1 reduced the release of lysosomal β-hexosaminidase. Vacuolin-1 also blocked the ionomycin-induced, Ca2+-dependent cell surface appearance of the luminal epitope from the lysosomal membrane protein, Lamp-1, a marker for fusion between the limiting membranes of lysosomes and the cell surface. Thus, vacuolin-1 blocks the Ca2+-dependent fusion of lysosomes with the plasma membrane and the release of lysosomal contents. While vacuolin-1 had no effect on the fusion of enlargeosomes with the plasma membrane. Other cell structures and membrane trafficking functions were also unaffected [1].

Reference:
[1].  Cerny J, Feng Y, Yu A, et al. The small chemical vacuolin-1 inhibits Ca2+-dependent lysosomal exocytosis but not cell resealing. EMBO Rep. 2004 Sep;5(9):883-8.

文献引用

化学属性

Physical AppearanceA crystalline solid
StorageStore at -20°C
M.Wt577.4
Cas No.351986-85-1
FormulaC26H24IN7O
Solubility≥7.28 mg/mL in DMSO with ultrasonic; insoluble in EtOH; insoluble in H2O
Chemical Name3-iodo-2-[4-(diphenylamino)-6-(4-morpholinyl)-1,3,5-triazin-2-yl]hydrazone, benzaldehyde
SDFDownload SDF
Canonical SMILESIC1=CC(/C=N/NC2=NC(N(C3=CC=CC=C3)C4=CC=CC=C4)=NC(N5CCOCC5)=N2)=CC=C1
运输条件 蓝冰运输或根据您的需求运输。
一般建议不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。

试验操作

Cell experiment:[1]

Cell lines

HeLa cells

Reaction Conditions

1, 5 or 10 μM vacuolin-1 for 1, 2 or 4 h incubation

Applications

In the absence of vacuolin-1, treatment of HeLa cells for 10 min with 5 μM ionomycin resulted in the expected release of 18 ~ 20% of lysosomal β-hexosaminidase. In contrast, cells that were pretreated with 5 or 10 μM vacuolin-1 for 2 h released no more β-hexosaminidase compared with cells that were not exposed to ionomycin (~ 4%). However, pretreatment of HeLa cells with 1 μM vacuolin-1 had no effect on the cell surface appearance of the enlargeosome marker in response to ionomycin. Thus, the inhibitory effect of vacuolin-1 on Ca2+-dependent exocytosis was not a general phenomenon. Rather, it seemed to be a specific effect for endosomes and lysosomes but not for enlargeosomes.

Note

The technical data provided above is for reference only.

References:

1. Cerny J, Feng Y, Yu A, et al. The small chemical vacuolin-1 inhibits Ca(2+)-dependent lysosomal exocytosis but not cell resealing. EMBO Reports, 2004, 5(9): 883-888.

质量控制