STF 31
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
STF-31 is a selective inhibitor of glucose transporter 1 (GLUT1), with an IC50 of 1 μΜ [1].
The inactivated von Hippel-Lindau (VHL) tumor suppressor gene drives renal cell carcinomas (RCCs) development, which imparts the reliance on the GLUT1 and aerobic glycolysis. Compounds of the 3-series, exemplified by STF-31, selectively kills RCCs by specifically targeting glucose uptake through GLU1, on which is the unique dependence of these cells for survival.
STF-31 (0.01-10 μΜ; 10 days) is specifically toxic to RCC4 cells without VHL, while wild-type RCC4 cells (RCC4/VHL) are relatively unaffected. Wild-type RCC4 cells treated with STF-31 (5 μΜ; 10 days) largely recovery, while RCC4 cells dose not on the same condition. No evidence proved the drug-induced cells autophagy, apoptosis, or DNA damages, but wild-type RCC4 cells underwent necrotic cell death in response to STF-31 by the ability of the cells to exclude trypan blue [1].
Soluble analog of STF-31 (11.6 mg/kg; i.p.) treatment delayed tumor growth in mice with VHL-deficient RCC tumor xenografts [1]. STF-31 (10 mg/kg; i.p.; twice daily for 2 days, followed by once daily for another 3 days) did not affect normal mice body weight, behavior and Scotopic electroretinography (ERG) responses. STF-31 reduced light-induced CX3CR1gfp/+ mice microglial activation and retinal degeneration [2].
Reference:
[1] Chan D A, Sutphin P D, Nguyen P, et al. Targeting GLUT1 and the Warburg effect in renal cell carcinoma by chemical synthetic lethality. Sci Transl Med. 2011, 3(94): 94ra70.
[2] Wang L X, Pavlou S, Du X, et al. Glucose transporter 1 critically controls microglial activation through facilitating glycolysis. Mol Neurodegener. 2019, 14(1): 2.
Physical Appearance | A solid |
Storage | Store at RT |
M.Wt | 423.53 |
Cas No. | 724741-75-7 |
Formula | C23H25N3O3S |
Solubility | ≥42.4 mg/mL in DMSO; insoluble in H2O; insoluble in EtOH |
Chemical Name | 4-((4-(tert-butyl)phenylsulfonamido)methyl)-N-(pyridin-3-yl)benzamide |
SDF | Download SDF |
Canonical SMILES | O=S(NCC1=CC=C(C(NC2=CC=CN=C2)=O)C=C1)(C3=CC=C(C=C3)C(C)(C)C)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
- 批次: