SR 11302
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
SR 11302是激活蛋白-1（AP-1）的抑制剂[1]。AP-1是体内具有抗肿瘤作用的转录因子。
SR 11302是AP-1的抑制剂，但不激活RAR和RXR。SR11302不能抑制F9胚胎癌细胞系的细胞增殖。它可以抑制乳腺癌细胞系T-47D、肺癌细胞系Calu-6和HeLa细胞的生长[1]。SR 11302对HL-60、新鲜的APL细胞和NB4细胞的增殖或分化影响较小，这表明AP-1不参与这些细胞的细胞增殖和分化信号传导途径[2]。
在AP-1萤光素酶转基因小鼠癌变模型中， 7,12-dimethyl benz(a)anthracene-initiated引发的小鼠皮肤和12-O-tetradecanoylphorbol-13-acetate诱导的乳头状瘤的形成中，SR11302显著抑制AP-1的活性。同时，SR11235是具有罕见的转录激活活性的类视黄酸，缺乏AP-1的抑制效果，不抑制乳头状瘤形成和AP-1的活化。这些结果表明，类视黄酸通过阻断体内AP-1的活性介导抗肿瘤效果[3]。
参考文献：
[1]. Fanjul A, Dawson MI, Hobbs PD, et al. A new class of retinoids with selective inhibition of AP-1 inhibits proliferation. Nature, 1994, 372(6501): 107-111.
[2]. Shiohara M, Dawson MI, Hobbs PD, et al. Effects of novel RAR- and RXR-selective retinoids on myeloid leukemic proliferation and differentiation in vitro. Blood, 1999, 93(6): 2057-2066.
[3]. Huang C, Ma WY, Dawson MI, et al. Blocking activator protein-1 activity, but not activating retinoic acid response element, is required for the antitumor promotion effect of retinoic acid. Proc Natl Acad Sci U S A, 1997, 94(11): 5826-5830.
- 1. Zhiwu Jiang, Le Qin, et al. "Human induced-T-to-natural killer cells have potent anti-tumour activities." Biomark Res. 2022 Mar 24;10(1):13. PMID: 35331335
- 2. Tong Wang, Qi Sun, et al. "A1 Reactive Astrocytes Activated by 2-chloroethanol Modulate M1 Microglia Polarization through Upregulating IL-1β and TNF-α." Research Square. April 13th, 2021
- 3. Tong Wang, Qi Sun, et al. "A1 astrocytes activated by 2-Chloroethanol via the ROS-induced p38 MAPK/NF-κB and AP-1 pathways modulate microglia polarization." Research Square. April 12th, 2021.
- 4. Lingyu Wang, Zizhen Gong, et al. "Gut microbial bile acid metabolite skews macrophage polarization and contributes to high-fat diet-induced colonic inflammation." Gut Microbes. 2020 Nov 9;12(1):1-20. PMID: 33006494
- 5. Weiler J, Dittmar T. "Minocycline impairs TNF-α-induced cell fusion of M13SV1-Cre cells with MDA-MB-435-pFDR1 cells by suppressing NF-κB transcriptional activity and its induction of target-gene expression of fusion-relevant factors." Cell Commun Signal. 2019 Jul 2;17(1):71. PMID: 31266502
- 6. Ding RR, Yuan JL, et al. "Epstein-Barr virus-encoded LMP1 regulated Pim1 kinase expression promotes nasopharyngeal carcinoma cells proliferation." Onco Targets Ther. 2019 Feb 11;12:1137-1146. PMID: 30809095
- 7. Wang T, Jin X, et al."Association of NF-κB and AP-1 with MMP-9 Overexpression in 2-Chloroethanol Exposed Rat Astrocytes." Cells. 2018 Aug 7;7(8). pii: E96. PMID: 30087244
- 8. Michael John Vanden Oever. "Regulation of Type VII Collagen in Patients with Recessive Dystrophic Epidermolysis Bullosa." UNIVERSITY OF MINNESOTA. 2017.
| Physical Appearance | A crystalline solid |
| Storage | Store at -20°C |
| M.Wt | 376.54 |
| Cas No. | 160162-42-5 |
| Formula | C26H32O2 |
| Solubility | Soluble in DMSO |
| Chemical Name | 3-methyl-7-(4-methylphenyl)-9-(2,6,6-trimethylcyclohexen-1-yl)nona-2,4,6,8-tetraenoic acid |
| SDF | Download SDF |
| Canonical SMILES | CC1=C(C(CCC1)(C)C)C=CC(=CC=CC(=CC(=O)O)C)C2=CC=C(C=C2)C |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 细胞实验[1]: | |
|
细胞系 |
T-47D，Calu-6和HeLa细胞系 |
|
溶解方法 |
在DMSO中的溶解度> 10 mM。为了获得更高浓度，可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。 |
|
反应条件 |
10-6 M |
|
应用 |
SR 11302可有效抑制T-47D，Calu-6和HeLa细胞的增殖，可作为新的低副作用的类视黄醇治疗剂的候选者。 |
| 动物实验[2]: | |
|
动物模型 |
AP-1-荧光素酶转基因小鼠 |
|
剂量 |
34nmol溶解于300μl丙酮中，连续7天，每天4次 |
|
应用 |
SR 11302可以通过阻断小鼠体内AP-1的活性，从而介导抗肿瘤效果，是化疗和化学预防癌症最有希望的候选药物之一。 |
|
注意事项 |
请测试所有化合物在室内的溶解度，实际溶解度和理论值可能略有不同。这是由实验系统的误差引起的，属于正常现象。 |
|
References: [1] Fanjul A, Dawson MI, Hobbs PD, et al. A new class of retinoids with selective inhibition of AP-1 inhibits proliferation. Nature, 1994, 372(6501): 107-111. [2] Huang C, Ma WY, Dawson MI, et al. Blocking activator protein-1 activity, but not activating retinoic acid response element, is required for the antitumor promotion effect of retinoic acid. Proc Natl Acad Sci U S A, 1997, 94(11): 5826-5830. |
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化学结构
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