Sanguinarine chloride
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Sanguinarine chloride是一种强效的和特异性的PP2C抑制剂,Ki值为0.68 μM,也是一种选择性的具细胞活性的MKP-1抑制剂,IC50值为10 μM.Sanguinarine也是一种AMPK变构激活剂[1] [2] [3].
蛋白磷酸酶2C(PP2C)是一种丝氨酸/苏氨酸特异性磷酸酶,其活性依赖于Mg2+或Mn2+.PP2C去磷酸化许多底物,如细胞周期蛋白依赖性激酶\促分裂原活化激酶(MAPK)和Bad.丝裂原活化蛋白激酶磷酸酶-1(MKP-1)是一种双重特异性磷酸酶.AMP活化蛋白激酶(AMPK)在细胞代谢的调节中起重要作用[1] [2] [3].
Sanguinarine chloride是一种PP2C和MKP-1抑制剂,并且还是一种AMPK变构激活剂,具有抗生素和抗肿瘤活性.Sanguinarine与α酪蛋白竞争抑制PP2C,并且与PP1\PP2A和PP2B相比表现出PP2C选择性.在人类早幼粒细胞白血病细胞系HL60中,Sanguinarine表现出细胞毒性,IC 50值为0.37 μM,并且通过caspase-3/7-依赖性机制诱导细胞凋亡,该机制有PP2C底物p38磷酸化的参与[1].Sanguinarine抑制MKP-1和MKP-L,IC50值分别为17.3和12.5 μM.在PANC-1人胰腺癌细胞中,Sanguinarine增加ERK和JNK/SAPK磷酸化[2].在MDA MB-231细胞系中,Sanguinarine引起AMPK和下游乙酰-CoA羧化酶(ACC)的磷酸化[3].在LNCaP和DU145细胞中,Sanguinarine抑制细胞生长,诱导G0 / G1期阻滞和细胞凋亡[4].
参考文献：
[1]. Aburai N, Yoshida M, Ohnishi M, et al. Sanguinarine as a potent and specific inhibitor of protein phosphatase 2C in vitro and induces apoptosis via phosphorylation of p38 in HL60 cells. Biosci Biotechnol Biochem, 2010, 74(3): 548-552.
[2]. Vogt A, Tamewitz A, Skoko J, et al. The benzo[c]phenanthridine alkaloid, sanguinarine, is a selective, cell-active inhibitor of mitogen-activated protein kinase phosphatase-1. J Biol Chem, 2005, 280(19): 19078-19086.
[3]. Choi J, He N, Sung MK, et al. Sanguinarine is an allosteric activator of AMP-activated protein kinase. Biochem Biophys Res Commun, 2011, 413(2): 259-263.
[4]. Adhami VM, Aziz MH, Reagan-Shaw SR, et al. Sanguinarine causes cell cycle blockade and apoptosis of human prostate carcinoma cells via modulation of cyclin kinase inhibitor-cyclin-cyclin-dependent kinase machinery. Mol Cancer Ther, 2004, 3(8): 933-940.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 367.78 |
| Cas No. | 5578-73-4 |
| Formula | C20H14ClNO4 |
| Solubility | insoluble in H2O; insoluble in EtOH; ≥3.68 mg/mL in DMSO with gentle warming |
| Chemical Name | 13-methyl-[1,3]dioxolo[4',5':4,5]benzo[1,2-c][1,3]dioxolo[4,5-i]phenanthridin-13-ium chloride |
| SDF | Download SDF |
| Canonical SMILES | C[N+]1=C2C(C=CC3=CC(OCO4)=C4C=C32)=C5C=CC(OCO6)=C6C5=C1.[Cl-] |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
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化学结构
