Ro 08-2750
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC50：1 μM（结合NGF）[1]
Ro 08-2750是一种神经生长因子（NGF）拮抗剂，其选择性抑制结合p75NTR优于TRKA147。这种选择性被用来研究p75NTR和TrkA在细胞凋亡、神经元分化和轴突生长中的功能。NGF是一种神经肽，主要参与调节某些目标神经元的生长、增殖、维持和存活。
体外：Ro 08-2750可以结合NGF二聚体，结合比约为1：1。Ro 08-2750结合NGF可诱导NGF浓度依赖性的构象变化[1]。与低浓度的RO 08-2750结合，NGF不能再结合p75NTR，但结合TrkA不受损。Ro 08-2750在超过5 mM的较高浓度下，NGF/RO 08-2750复合物也不能结合TrkA。在SK-N-MC细胞中，Ro 08-2750导致NGF诱导的程序性细胞死亡的抑制。在PC12细胞中，Ro 08-2750可以通过TrkA增强信号通路[2]。
体内：目前尚未进行体内研究。
临床试验：目前尚未进行临床研究。
参考文献：
[1] Arkin MR, Wells JA. Small-molecule inhibitors of protein-protein interactions: progressing towards the dream. Nat Rev Drug Discov. 2004 Apr;3(4):301-17.
[2] Niederhauser O, Mangold M, Schubenel R, Kusznir EA, Schmidt D, Hertel C. NGF ligand alters NGF signaling via p75(NTR) and trkA. J Neurosci Res. 2000 Aug 1;61(3):263-72.
| Physical Appearance | Orange solid |
| Storage | Store at -20°C |
| M.Wt | 270.24 |
| Cas No. | 37854-59-4 |
| Formula | C13H10N4O3 |
| Solubility | ≤2.7mg/ml in DMSO |
| Chemical Name | 7,10-dimethyl-2,4-dioxo-2,3,4,10-tetrahydrobenzo[g]pteridine-8-carbaldehyde |
| SDF | Download SDF |
| Canonical SMILES | O=C1NC(N=C2N(C)C3=CC(C=O)=C(C)C=C3N=C21)=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
化学结构
相关生物数据
相关生物数据
