R-7050
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
EC50: 0.631 μM by TNF-α by ICAM-1 ELISA assay
R-7050 is a TNF-α receptor signaling antagonist.
TNF-α induces biological activity throgh stimulation of the tumor necrosis factor receptor. Although plenty of pre-clinical and clinical evidence suggests a detrimental role, small molecule TNF-αsignaling pathway inhibitors remain largely unexplored in the context of a brain hemorrhage.
In vitro: R-7050 was identified as a selective inhibitot of TNF-α induced cellular signaling by using differential library screening. Unlike biologic TNF inhibitors directly binding TNF-α, R-7050 could not affect binding of TNF-α to TNFR. In contrast, R-7050 was able to selectively inhibit the association of TNF receptor with intracellular adaptor molecules [1].
In vivo: In previous animal study, R-7050 was tested to attenuate neurovascular injury after Intracerebral hemorrhage (ICH) in mice. Results showed that up to 2h post-injury administration of R-7050 was able to significantly reduce blood-brain barrier opening and attenuate edema development at 24h post-ICH. In addition, neurological outcomes were also improved over the first 3 days after injury. Whereas, R-7050 was not found to be albe to reduce hematoma volume, indicating the beneficial effects of TNFR inhibition were downstream of clot formation/resolution [2].
Clinical trial: Up to now, R-7050 is still in the preclinical development stage.
References:
[1] Gururaja TL, Yung S, Ding R, Huang J, Zhou X, McLaughlin J, Daniel-Issakani S, Singh R, Cooper RD, Payan DG, Masuda ES, Kinoshita T. A class of small molecules that inhibit TNFalpha-induced survival and death pathways via prevention of interactions between TNFalphaRI, TRADD, and RIP1. Chem Biol. 2007 Oct;14(10):1105-18.
[2] King MD, Alleyne CH Jr, Dhandapani KM. TNF-alpha receptor antagonist, R-7050, improves neurological outcomes following intracerebral hemorrhage in mice. Neurosci Lett. 2013 May 10;542:92-6.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 380.8 |
Cas No. | 303997-35-5 |
Formula | C16H8ClF3N4S |
Synonyms | TNF-α Antagonist III |
Solubility | insoluble in H2O; ≥17.67 mg/mL in DMSO; ≥2.2 mg/mL in EtOH with gentle warming and ultrasonic |
Chemical Name | 8-chloro-4-(phenylthio)-1-(trifluoromethyl)-[1,2,4]triazolo[4,3-a]quinoxaline |
SDF | Download SDF |
Canonical SMILES | ClC1=CC(N2C3=NN=C2C(F)(F)F)=C(C=C1)N=C3SC4=CC=CC=C4 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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