2-Bromohexadecanoic acid

2‑Bromohexadecanoic acid（CAS号：18263-25-7）是常用的“抑制蛋白S‑棕榈酰化”工具化合物。其在细胞内可转化为2‑bromopalmitoyl‑CoA并发生共价反应，广谱抑制DHHC（(Asp-His-His-Cys)）家族棕榈酰转移酶（PAT），不仅影响膜定位/信号转导，也可在特定情境下抑制 BAK/BAX‑caspase‑3‑GSDME 通路介导的焦亡（化疗条件下GSDME‑C发生棕榈酰化促进BAK/BAX‑caspase‑3‑GSDME介导的焦亡（pyroptosis），而2‑BP可抑制GSDME‑C棕榈酰化并阻断焦亡）。体外纯化酶实验中，2‑BP对人源zDHHC20的IC₅₀约2.02±0.29 µM（另有acyl‑cLIP法约5.33±0.77 µM）；但其靶标广泛、非选择性，建议结合遗传学/正交方法验证机制。

参考文献：

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