Buthionine Sulfoximine
| 规格 | 价格 | 货期 | 数量 |
|---|---|---|---|
| 250mg | ￥909.00 | 现货 | |
| 500mg | ￥1727.00 | 现货 | |
| 1g | ￥3272.00 | 现货 |
特色产品
- 适用于流式细胞仪和荧光显微镜检测
- 准确区分活细胞/凋亡细胞/坏死细胞
- 用于免疫印迹和质谱分析等后续操作
- 适用于30 KDa-130 KDa大小的蛋白
- 对细胞无毒性
- 步骤更简单，无需有机溶剂
- 可将信号灵敏度提高100倍
- 同时保持稳定的特异性和分辨率
- 提供更高的转录效率并抑制免疫激活
- 使用5-moUTP和Cy5-utp修饰
- 优异的扩增效率、可重复性和稳定性
- 卓越的灵敏度和准确性，操作简单
- 优化的缓冲液和T7 RNA聚合酶混合物
- 20 μL反应体系可获得更高的RNA产量
产品描述
L-Buthionine-(S,R)-sulfoximine is a cell-permeable, irreversible inhibitor of g-glutamylcysteine synthetase[1].
In ZAZ and M14 melanoma cell line, L-Buthionine-(S,R)-sulfoximine (50 μM) treatment for 48 hr results in a 95% decrease in GSH levels, and a 60% decrease in GST enzyme activity. GST-mu protein and mRNA levels are significantly reduced in both cell lines[1].
L-Buthionine-(S,R)-sulfoximine (BSO) treatment significantly increases the frequency of DNA deletions and reduces concentrations of GSH and cysteine. BSO (20 mM) treatment results in a 40% increase in DNA deletion frequency, a 70% decrease in GSH, and a 55% decrease in cysteine levels[2].
References：
[1]. Fruehauf J P, et al. Selective and synergistic activity of L-S,R-buthionine sulfoximine on malignant melanoma is accompanied by decreased expression of glutathione-S-transferase. Pigment Cell Res, 1997, 10(4): 236-49.
[2]. Reliene R, et al. Glutathione depletion by buthionine sulfoximine induces DNA deletions in mice. Carcinogenesis, 2006, 27(2): 240-4.
产品性质
| 物理外观 | A crystalline solid |
| CAS号 | 83730-53-4 |
| 分子式 | C8H18N2O3S |
| 分子量 | 222.31 |
| 小分子别名 | L-Buthionine-(S,R)-sulfoximine |
| 化学名称 | (2S)-2-amino-4-(butylsulfonimidoyl)butanoic acid |
| 溶解度 | insoluble in DMSO; insoluble in EtOH; ≥26.15 mg/mL in H2O |
| SMILES | O=S(CCCC)(CC[C@@H](C(O)=O)N)=N |
| 存储条件 | -20°C |
| 运输条件 | 蓝冰 |
产品应用 (实验数据来自文献，APExBIO并未验证，仅供参考)
IC50和靶点
| 生物活性描述 | L-Buthionine-(S,R)-sulfoximine 是一种具有细胞渗透性、强效、快速作用且不可逆的 g-谷氨酰半胱氨酸合成酶抑制剂；可消耗细胞中的谷胱甘肽水平。L-Buthionine-(S,R)-sulfoximine 对黑色素瘤、乳腺癌和卵巢癌标本的 IC50 值分别为 1.9 μM、8.6 μM 和 29 μM。 |
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