Niclosamide
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Niclosamide is an inhibitor of the signal transducer and activator of transcription 3 (STAT3) with IC50 value of 0.7 μM [1].
STATs are a class of transcription factors that regulate fundamental cellular and biological processes, such as cell proliferation, cell survival, immune responses, and angiogenesis, by modulating the expression of specific target genes. STAT3 has been frequently reported to be over-activated in various human cancer types [1].
In HeLa cells, Niclosamide (< 5 μM) inhibited STAT3-mediated luciferase reporter activity with in a dose dependent manner, with IC50 value of 0.25 μM. In Du145 cancer cells, Niclosamide(< 10 μM) dose-dependently induced G0/G1 arrest and apoptosis [1].
In nude mice bearing HL-60 xenograft tumors, Niclosamide treatment (40 mg/kg/d, i.p.) for 15 days potently inhibited the growth of HL-60 tumors. Further, immunoblotting of xenograft tissues from mice revealed a potent inhibitory effect of Niclosamide on the pathway of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) [2].
References:
[1]. Ren X, Duan L, He Q, et al. Identification of Niclosamide as a New Small-Molecule Inhibitor of the STAT3 Signaling Pathway. ACS Medicinal Chemistry Letters, 2010, 1(9): 454-459.
[2]. Jin Y, Lu Z, Ding K, et al. Antineoplastic mechanisms of niclosamide in acute myelogenous leukemia stem cells: inactivation of the NF-kappaB pathway and generation of reactive oxygen species. Cancer Research, 2010, 70(6): 2516-2527.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 327.12 |
Cas No. | 50-65-7 |
Formula | C13H8Cl2N2O4 |
Solubility | insoluble in H2O; ≥12.75 mg/mL in EtOH with gentle warming; ≥8.2 mg/mL in DMSO with gentle warming and ultrasonic |
Chemical Name | 5-chloro-N-(2-chloro-4-nitrophenyl)-2-hydroxybenzamide |
SDF | Download SDF |
Canonical SMILES | C1=CC(=C(C=C1[N+](=O)[O-])Cl)NC(=O)C2=C(C=CC(=C2)Cl)O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
Cell experiment:[1] | |
Cell lines |
Du145 prostate cancer cells |
Reaction Conditions |
0 ~ 10 μM niclosamide for 24 h incubation |
Applications |
Niclosamide (≤ 2 μM) dose-dependently inhibited the Tyr-705 phosphorylation of STAT3 and the transcription of STAT3 downstream genes in Du145 cells. In addition, niclosamide induced G0/G1 arrest (≤ 1 μM) and apoptosis of Du145 cells (≤ 10 μM) in a dose dependent manner. |
Animal experiment:[2] | |
Animal models |
Nude mice bearing HL-60 xenograft tumors |
Dosage form |
40 mg/kg/d Injected intraperitoneally (i.p.) for 15 days |
Applications |
In nude mice bearing HL-60 xenograft tumors, niclosamide treatment (40 mg/kg/d, i.p.) for 15 days potently inhibited the growth of HL-60 tumors. Further, immunoblotting of xenograft tissues from mice revealed a potent inhibitory effect of niclosamide on the NF-κB pathway. |
Note |
The technical data provided above is for reference only. |
References: 1. Ren X, Duan L, He Q, et al. Identification of Niclosamide as a New Small-Molecule Inhibitor of the STAT3 Signaling Pathway. ACS Medicinal Chemistry Letters, 2010, 1(9): 454-459. 2. Jin Y, Lu Z, Ding K, et al. Antineoplastic mechanisms of niclosamide in acute myelogenous leukemia stem cells: inactivation of the NF-kappaB pathway and generation of reactive oxygen species. Cancer Research, 2010, 70(6): 2516-2527. |
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