Neutrophil Elastase Inhibitor
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC50: 7 nM
Neutrophil Elastase Inhibitor is a neutrophil elastase inhibitor.
Human neutrophil elastase (HNE) is a member of the chymotrypsin superfamily of serine proteases that is involved in the response to inflammatory stimuli. HNE is reported to be stored in azurophilic granules of neutrophils and is greatly aggressive and cytotoxic, as its substrates include various components of the extracellular matrix.
In vitro: Previous study found that Neutrophil Elastase Inhibitor was able to inhibit thrombin and urokinase at micromolar concentrations. In addition, Neutrophil Elastase Inhibitor appeared to be a specific HNE inhibitors in a competitive manner. Moreoer, the hydrolysis of the acyl-enzyme complex for Neutrophil Elastase Inhibitor was found to be much slower than spontaneous hydrolysis of Neutrophil Elastase Inhibitor, and HNE was observed to be still inhibited to 80% of control level at 7 h after treatment [1].
In vivo: So far, there is no animal in vivo data reported.
Clinical trial: Up to now, Neutrophil Elastase Inhibitor is still in the preclinical development stage.
References:
[1] L. Crocetti, I. A. Schepetkin, A. Cilibrizzi, et al. Optimization of N-benzoylindazole derivatives as inhibitors of human neutrophil elastase. Journal of Medicinal Chemistry 56(15), 6259-6272 (2013).
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 261.3 |
Cas No. | 1448314-31-5 |
Formula | C16H11N3O |
Solubility | ≤0.5mg/ml in ethanol;10mg/ml in DMSO;30mg/ml in dimethyl formamide |
Chemical Name | 1-(3-methylbenzoyl)-1H-indazole-3-carbonitrile |
SDF | Download SDF |
Canonical SMILES | O=C(C1=CC=CC(C)=C1)N2C3=CC=CC=C3C(C#N)=N2 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
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