N-Oxalylglycine
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC50: 250 μM: inhibits α-ketoglutarate-dependent enzyme jumonji domain-containing protein 2 (JMJD2) A.
IC50: 500 μM: blocks α-ketoglutarate-dependent enzyme JMJD2C.
IC50: 24 μM: dampens α-ketoglutarate-dependent enzyme JMJD2E.
IC50: 2.1 μM: suppresses the prolyl hydroxylase domain-containing proteins PHD1.
IC50: 5.6 μM: depresses the prolyl hydroxylase domain-containing proteins PHD2.
N-Oxalylglycine, as known as NOG, is a cell permeable inhibitor of α-ketoglutarate-dependent enzymes which include JMJD2A, JMJD2C, and JMJD2E. NOG also blocks the prolyl hydroxylase domain-containing proteins PHD1 and PHD2. Demethylation of the methylated forms of histone 3 lysine 9 (H3K9) and H3K36 can be catalyzed by JMJD2 subfamily of histone demethylases. Since histone demethylases are related to certain diseases, including cancer, selective inhibitors functions as anticancer agents and potential tools for clarifying the biological functions of JMJDs.
In vitro: NOG was identified as a natural product and presented in plants, including Rheum rhabarbarum (rhubarb) and Spinach oleracea (spinach) leaves. However, there was no NOG observed human embryonic kidney cells (HEK 293T) or Escherchia coli. NOG regulated gene expression via blocking 2-Oxoglutarate-dependent oxygenases [1].
In vivo: Up to now, in vivo study of NOG is still in the development stage.
Reference:
[1]. Al-Qahtani, K., Jabeen, B., Sekirnik, R., Riaz, N., Claridge, T., Schofield, C., & McCullagh, J. The broad spectrum 2-oxoglutarate oxygenase inhibitor N-oxalylglycine is present in rhubarb and spinach leaves. Phytochemistry. 2015; 117: 456-461.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 147.1 |
Cas No. | 5262-39-5 |
Formula | C4H5NO5 |
Synonyms | NOG |
Solubility | insoluble in EtOH; ≥3.82 mg/mL in DMSO with ultrasonic; ≥9.55 mg/mL in H2O |
Chemical Name | N-(carboxycarbonyl)-glycine |
SDF | Download SDF |
Canonical SMILES | OC(C(NCC(O)=O)=O)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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