Methyl α-Linolenyl Fluorophosphonate
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Methyl α-Linolenyl Fluorophosphonate is an inhibitor of phospholipases.
Methyl α-linolenyl fluorophosphonate (MLnFP), an analog of methyl arachidonyl fluorophosphonate (MAFP), has not been completely investigated for its pharmacological activity.
In vitro: Methyl arachidonyl fluorophosphonate (MAFP) was reported to be a selective, active-site directed, irreversible inhibitor of cytosolic phospholipase A2 (cPLA2). MAFP could also potently inhibit the Ca2+-independent cytosolic phospholipase A2 (iPLA2) in a concentration-dependent manner. Such inhibition was not reversed upon extensive dilution of the enzyme into the assay mixture. Preincubation of iPLA2 with MAFP led to a linear and time-dependent inactivation of enzyme activity, and the enzyme was protected from inactivation by the reversible inhibitor. The ability of MAFP to inhibit the iPLA 2 indicated that this enzyme proceeded via an acyl-enzyme intermediate. Further testing showed that MAFP could not inhibit the CoA-dependent acyltransferase, arachidonoyI-CoA synthetase, or CoA-independent transacylase activities. Therefore, MAFP was not a general inhibitor for enzymes acting on arachidonoyi substrates [1].
In vivo: Up to now, there is no animal in vivo data reported.
Clinical trial: So far, no clinical study has been conducted.
Reference:
[1] Lio, Y. C.,Reynolds, L.J.,Balsinde, J., et al. Irreversible inhibition of Ca2+-independent phospholipase A2 by methyl arachidonyl fluorophosphonate. Biochimica et Biophysica Acta 1302, 55-60 (1996).
Physical Appearance | A solution in acetate. To change the solvent, simply evaporate the acetate containing under a gentle stream of nitrogen and immediately add the solvent of choice. |
Storage | Store at -20°C |
M.Wt | 344.4 |
Formula | C19H34FO2P |
Synonyms | MLnFP |
Solubility | ≤5mg/ml in ethanol;5mg/ml in DMSO;5mg/ml in dimethyl formamide |
Chemical Name | 9Z,12Z,15Z-octadecatrienyl-phosphonofluoridic acid, methyl ester |
SDF | Download SDF |
Canonical SMILES | CC/C=C\C/C=C\C/C=C\CCCCCCCCP(=O)(F)OC |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |