L-NIO (hydrochloride)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC50: 1.7, 3.9, and 3.9 μM for nNOS (rat), eNOS (bovine), and iNOS (mouse), respectively
L-NIO is a NOS inhibitor.
Nitric oxide synthases (NOSs) are enzymes that catalyze the production of nitric oxide (NO) from L-arginine. NO is an important cellular signaling molecule and helps modulate vascular tone, airway tone, insulin secretion, and peristalsis. NO is involved in angiogenesis and neural development.
In vitro: Three analogues of L-arginine were characterized as inhibitors of endothelial NO synthase by measuring their effect on the endothelial NO synthase from porcine aortae. It was found that L-NMMA, L-NIO and L-NAME could cause concentration-dependent inhibition of the Ca2+-dependent endothelial NO synthase [1].
In vivo: In rats, L-NMMA, L-NIO and L-NAME could induce a dose-dependent increase in mean systemic arterial blood pressure accompanied by bradycardia. Moreover, L-NMMA, L-NIO and L-NAME at i.v. 100 mg/kg significantly inhibited the hypotensive responses to ACh and bradykinin. The increase in blood pressure and bradycardia produced by these compounds were able to be reversed by L-arginine in a dose-dependent manner. Moreover, all of these effects were enantiomer specific [1].
Clinical trial: So far, no clinical study has been conducted.
Reference:
[1] D. D. Rees, R. M. J. Palmer, R. Schulz, et al. Characterization of three inhibitors of endothelial nitric oxide synthase in vitro and in vivo. British Journal of Pharmacology 101, 746-752 (1990).
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 246.1 |
Cas No. | 36889-13-1 |
Formula | C7H15N3O2·2HCl |
Solubility | ≤20mg/ml in ethanol;20mg/ml in DMSO;20mg/ml in dimethyl formamide |
Chemical Name | N5-(1-iminoethyl)-L-ornithine, dihydrochloride |
SDF | Download SDF |
Canonical SMILES | CC(=N)NCCC[C@H](N)C(=O)O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |