K145
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
K145是鞘氨醇激酶2(SphK2)的选择性抑制剂,其IC50值为4.3 μM[1].
K145是一种鞘氨醇类似物,与SphK2底物竞争,体外测定Ki值为6.4 μM.相对于SphK1和CERK,K145选择性地抑制SphK2.在U937细胞中,K145降低细胞总S1P并抑制FTY720磷酸化(S1P与FTY720均为SphK2的底物).同时,K145显著抑制细胞生长并诱导晚期凋亡.在上述细胞系中,K145也能抑制ERK和Akt磷酸化[1].
在U937细胞异种移植小鼠模型中,K145抑制肿瘤生长,其TGI值为44.2%,较Tamibarotene毒性小.在JC异种移植BALB/c小鼠中,K145也能有效抑制肿瘤生长.此外,在U937细胞异种移植BALB/C-nu小鼠中,口服K145比Tamibarotene具有更好的抗肿瘤活性[1].
参考文献：
[1] Liu K, Guo T L, Hait N C, et al. Biological characterization of 3-(2-amino-ethyl)-5-[3-(4-butoxyl-phenyl)-propylidene]-thiazolidine-2, 4-dione (K145) as a selective sphingosine kinase-2 inhibitor and anticancer agent. PloS one, 2013, 8(2): e56471.
| Storage | Store at -20°C |
| M.Wt | 348.46 |
| Cas No. | 1309444-75-4 |
| Formula | C18H24N2O3S |
| Solubility | Soluble in DMSO |
| Chemical Name | (Z)-3-(2-aminoethyl)-5-(3-(4-butoxyphenyl)propylidene)thiazolidine-2,4-dione |
| SDF | Download SDF |
| Canonical SMILES | CCCCOC1=CC=C(C=C1)CC/C([H])=C(S2)/C(N(C2=O)CCN)=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
化学结构
