INH6
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
INH6是一种有效的Hec1/Nek2抑制剂[1].
Hec1是一种原癌基因,参与纺锤体检验点信号,在多种人类癌症中过表达.Nek2是一种丝氨酸/苏氨酸蛋白激酶,可磷酸化Hec1,这对其有丝分裂功能和细胞存活至关重要[1].
INH6是一种有效的Hec1/Nek2抑制剂.INH6可通过导致染色体错误分离和细胞死亡的蛋白降解,抑制Hec1/Nek2功能.在MDA-MB468和MDA-MB231人类乳腺癌细胞系\HeLa人类宫颈癌细胞系和K562人成红细胞性白血病细胞系中,INH6具有显著的抗增殖活性,IC50值分别为2.1\1.7\2.4和2.5 M.在HeLa细胞抽提物中,INH6耦联基质可选择性地与细胞Hec1共沉淀,表明INH6结合细胞Hec1.在Hela细胞中,INH6(6.25 M)可在8-11 h减少50%的Nek2.此外,INH6随时间进展可略微降低Hec1.另外,INH6可增加具有多极纺锤体配置的有丝分裂细胞数.在表达染色体标记蛋白H2B-GFP的HeLa细胞中,INH6可增加染色体错配.在HeLa细胞中,INH6可诱发垂死细胞发生积极的形态学变化,并诱导20%的细胞凋亡[1].
参考文献：
[1]. Qiu XL, Li G, Wu G, et al. Synthesis and biological evaluation of a series of novel inhibitor of Nek2/Hec1 analogues. J Med Chem, 2009, 52(6): 1757-1767.
| Storage | Desiccate at -20°C |
| M.Wt | 322.42 |
| Cas No. | 1001753-24-7 |
| Formula | C19H18N2OS |
| Solubility | ≥32.2 mg/mL in DMSO; insoluble in H2O; ≥3.55 mg/mL in EtOH with gentle warming and ultrasonic |
| SDF | Download SDF |
| Canonical SMILES | O=C(C1=CC=CC=C1)NC2=NC(C3=C(C)C=C(C)C=C3C)=CS2 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| Description | INH6是有效的Hec1抑制剂. | |||||
| 靶点 | Hec1/Nek2 complex | |||||
| IC50 | ||||||
质量控制和MSDS
- 批次:
化学结构
