Hemin chloride
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Hemin chloride is an oxidized form of heme that inhibits eukaryotic translation initiation factor 2α kinase 1 (eIF2αK1), a repressor of eIF-2α [1].
The eukaryotic initiation factor 1 (eIF1) plays an important role in translation start site specificity. The eIF2α is a key target in translational regulation. Phosphorylation of eIF2α on Ser51 converts eIF2 from a substrate to a competitive inhibitor of its exchange factor, eIF2β[2].
In vitro: The addition of hemin (30 μM) depressed the extent of incorporation of phosphate into eIF-2β by about 50% [1]. Concentrations of 25 μM hemin significantly decreased the rate of incorporation of phosphate into eIF-2α. Concentrations of hemin ranged from 20 to 40 μM maintained an optimal rate of protein synthesis in cell lysates [1].
In vivo: In mice, pretreated with an intraperitoneal injection of hemin displayed a marked induction of HO-1. In hemin-treated mice, thrombus formation was delayed, and the delay was completely blunted by tin protoporphyrin-IX [3].
References:
[1] Tahara S M, Traugh J A, Sharp S B, et al. Effect of hemin on site-specific phosphorylation of eukaryotic initiation factor 2[J]. Proceedings of the National Academy of Sciences, 1978, 75(2): 789-793.[]
[2] Sonenberg N, Dever T E. Eukaryotic translation initiation factors and regulators[J]. Current opinion in structural biology, 2003, 13(1): 56-63.
[3] Lindenblatt N, Bordel R, Schareck W, et al. Vascular heme oxygenase-1 induction suppresses microvascular thrombus formation in vivo[J]. Arteriosclerosis, thrombosis, and vascular biology, 2004, 24(3): 601-606
.Physical Appearance | A crystalline solid |
Storage | Store at 2-8°C |
M.Wt | 652 |
Cas No. | 16009-13-5 |
Formula | C34H30ClFeN4O4·2H |
Solubility | ≥26.5 mg/mL in DMSO; insoluble in EtOH; insoluble in H2O |
Chemical Name | (SP-5-13)-chloro[7,12-diethenyl-3,8,13,17-tetramethyl-21H,23H-porphine-2,8-dipropanoato(4-)-κN21, κN22, κN23, κN24]-ferrate(2-), dihydrogen |
SDF | Download SDF |
Canonical SMILES | [O-]C(CCC1=C(C=C2[N]3=C4C(C)=C2CCC([O-])=O)[N-]([Fe+3]35([Cl-])[N]6=C7C=C(C(C=C)=C8C)[N-]5C8=C4)C(C=C6C(C=C)=C7C)=C1C)=O.[H+].[H+] |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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