Dinactin
| 规格 | 价格 | 货期 | 数量 |
|---|---|---|---|
| 1mg | ￥6585.00 | 10-15工作日发货 | |
| 5mg | ￥22851.00 | 10-15工作日发货 |
特色产品
- 适用于流式细胞仪和荧光显微镜检测
- 准确区分活细胞/凋亡细胞/坏死细胞
- 用于免疫印迹和质谱分析等后续操作
- 适用于30 KDa-130 KDa大小的蛋白
- 对细胞无毒性
- 步骤更简单，无需有机溶剂
- 可将信号灵敏度提高100倍
- 同时保持稳定的特异性和分辨率
- 提供更高的转录效率并抑制免疫激活
- 使用5-moUTP和Cy5-utp修饰
- 优异的扩增效率、可重复性和稳定性
- 卓越的灵敏度和准确性，操作简单
- 优化的缓冲液和T7 RNA聚合酶混合物
- 20 μL反应体系可获得更高的RNA产量
产品描述
Dinactin is a macrotetrolide antibiotic that acts as an ionophore for monovalent cations, such as NH4+, K+, and Rb+. Dinactin has been used to study the membrane properties [1]. Dinactin is considered as an active molecule that S. araujoniae produced to control B. cinerea[2].
In vitro: Dinactin inhibited T-cell proliferation induced by IL-2, by mAb to CD3, and by mAbs to CD3 plus α-CD28 with identical dose-response curves. The IC50 was 10–20 ng/ml. Dinactin inhibited cytokine production with IC50 values of 10 ng/ml for IL-4 and IL-5, 30 or 60 ng/ml for interferong or IL-2, respectively [3].Dinactin inhibited cytokine production through a post-transcriptional mechanism. Dinactin also reduced pulmonary eosinophilia when administered within 1 d of airway antigen challenge [3].
References:
[1] Laprade R, Grenier F, Pagé-Dansereau M, et al. Carrier-mediated ion transport in lipid bilayer membranes[J]. Canadian journal of biochemistry and cell biology, 1984, 62(8): 738-751.
[2] Silva L J, Crevelin E J, Souza W R, et al. Streptomyces araujoniae produces a multiantibiotic complex with ionophoric properties to control Botrytis cinerea[J]. Phytopathology, 2014, 104(12): 1298-1305.
[3] Umland S P, Shah H, Jakway J P, et al. Effects of cyclosporin A and dinactin on T-cell proliferation, interleukin-5 production, and murine pulmonary inflammation[J]. American journal of respiratory cell and molecular biology, 1999, 20(3): 481-492.
产品性质
| 物理外观 | A film |
| CAS号 | 20261-85-2 |
| 分子式 | C42H68O12 |
| 分子量 | 765 |
| 化学名称 | (1R,2R,5R,7R,10S,11S,14S,16S,19R,20R,23R,25R,28S,29S,32S,34S)-5,23-diethyl-2,11,14,20,29,32-hexamethyl-4,13,22,31,37,38,39,40-octaoxapentacyclo[32.2.1.17,10.116,19.125,28]tetracontane-3,12,21,30-tetrone |
| 溶解度 | Soluble in DMSO |
| SMILES | O=C(O[C@H](CC)C[C@]1([H])O[C@@]([C@@H]2C)([H])CC1)[C@H](C)[C@@]3([H])CC[C@](C[C@H](C)OC([C@H]([C@@]4([H])O[C@](C[C@H](OC([C@H](C)[C@]5([H])O[C@](CC5)([H])C[C@H](C)OC2=O)=O)CC)([H])CC4)C)=O)([H])O3 |
| 存储条件 | -20°C |
| 运输条件 | 蓝冰 |
产品应用 (实验数据来自文献，APExBIO并未验证，仅供参考)
IC50和靶点
| 生物活性描述 | Dinactin是一种由链霉菌产生的离子诱导体，是一种有效的靶向细胞信号通路的小分子。Dinactin 能明显抑制 HCT-116 细胞的生长，其 IC 值为 1.1 µM。Dinactin 对细胞的抗增殖活性与细胞凋亡无关。Dinactin 还是研究神经性疼痛的有效药物。 |
质量控制
APExBIO 顾客使用本产品发表的 1 篇科研文献
- 1. Rawangkan A. "Dinactin: A New Antitumor Antibiotic with Cell Cycle Progression and Cancer Stemness Inhibiting Activities in Lung Cancer." Antibiotics (Basel) 2022 Dec 19;11(12) PMID: 36551502
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