DCPIB
| 规格 | 价格 | 货期 | 数量 |
|---|---|---|---|
| 10mg | ￥1006.00 | 现货 | |
| 50mg | ￥3625.00 | 现货 |
特色产品
- 适用于流式细胞仪和荧光显微镜检测
- 准确区分活细胞/凋亡细胞/坏死细胞
- 用于免疫印迹和质谱分析等后续操作
- 适用于30 KDa-130 KDa大小的蛋白
- 对细胞无毒性
- 步骤更简单，无需有机溶剂
- 可将信号灵敏度提高100倍
- 同时保持稳定的特异性和分辨率
- 提供更高的转录效率并抑制免疫激活
- 使用5-moUTP和Cy5-utp修饰
- 优异的扩增效率、可重复性和稳定性
- 卓越的灵敏度和准确性，操作简单
- 优化的缓冲液和T7 RNA聚合酶混合物
- 20 μL反应体系可获得更高的RNA产量
产品描述
DCPIB is a volume-regulated anion channel (VRAC) blocker (IC50 = 2 μM in rat pancreatic β-cells)[1], which is also a blocker of swelling-induced chloride current (ICl, swell) in various cardiovascular tissues (IC50 = 4.1 μM in CPAE cells)[2].
In intact β-cells, DCPIB inhibits glucose-stimulated insulin secretion through VSAC inhibition and indirect KATP channel activation. DCPIB reverses the shortened duration of action potential induced by cell swelling in atrial myocytes and inhibits astrocyte swelling in vitro. DCPIB (10 μM) also activates TREK1 and TRAAK K+ channels and inhibits TRESK, TASK1 and TASK3 K+ channels in vitro[3].
References:
[1]. Best L, Yates A P, Decher N, et al. Inhibition of glucose-induced electrical activity in rat pancreatic β-cells by DCPIB, a selective inhibitor of volume-sensitive anion currents. european journal of pharmacology, 2004, 489(1-2): 13-19.
[2].Decher N, et al. DCPIB is a novel selective blocker of I(Cl,swell) and prevents swelling-induced shortening of guinea-pig atrial action potential duration. Br J Pharmacol, 2001, 134(7): 1467-79.
[3]. Lv J, et al. DCPIB, an Inhibitor of Volume-Regulated Anion Channels, Distinctly Modulates K2P Channels. ACS Chem Neurosci, 2019.
产品性质
| 物理外观 | White solid |
| CAS号 | 82749-70-0 |
| 分子式 | C22H28Cl2O4 |
| 分子量 | 427.37 |
| 化学名称 | 4-((2-butyl-6,7-dichloro-2-cyclopentyl-1-oxo-2,3-dihydro-1H-inden-5-yl)oxy)butanoic acid |
| 溶解度 | insoluble in H2O; ≥49.8 mg/mL in EtOH; ≥50.8 mg/mL in DMSO |
| SMILES | CCCCC(Cc(cc1OCCCC(O)=O)c2c(Cl)c1Cl)(C1CCCC1)C2=O |
| 存储条件 | -20°C |
| 运输条件 | 蓝冰 |
产品应用 (实验数据来自文献，APExBIO并未验证，仅供参考)
IC50和靶点
| 生物活性描述 | DCPIB 是体积调节阴离子通道 (VRAC) 的一种选择性、可逆和强效抑制剂。DCPIB 可电压依赖性地激活钾通道 TREK1 和 TRAAK，并抑制 TRESK、TASK1 和 TASK3（IC50s 分别为 0.14、0.95 和 50.72 μM）。DCPIB 还是膨胀诱导的氯离子电流（ICl,swell）的选择性阻断剂，IC50 为 4.1 μM。DCPIB 是研究 K2P 通道结构-功能的有用工具。 |
生物相关数据
质量控制
操作说明
APExBIO 顾客使用本产品发表的 2 篇科研文献
- 1. Yuman Wang, Zaiqiao Sun, et al. "Cell volume controlled by LRRC8A-formed volume-regulated anion channels fine-tunes T cell activation and function." Nat Commun. 2023 Nov 4;14(1):7075. PMID: 37925509
- 2. Xiao-yan Wu, Jin-yan Lv, et al. "ML365 inhibits TWIK2 channel to block ATP-induced NLRP3 inflammasome." Acta Pharmacol Sin. 2022 Apr;43(4):992-1000. PMID:34341510
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