Cytochalasin D
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cytochalasin D是肌动蛋白聚合的选择性抑制剂,其IC50值为25 nM[1]。
肌动蛋白是一种球状的多功能蛋白,几乎存在于所有真核细胞中。实验表明,肌动蛋白聚合在趋化性和胞质分裂中起关键作用。据报道,Cytochalasin D在肌动蛋白聚合过程中起着抑制剂的作用,破坏肌动蛋白微丝以及激活p53依赖性途径,从而导致细胞周期阻滞在G1-S过渡期[2]。
在分化的神经元中,Cytochalasin D通过抑制肌动蛋白聚合,减慢伸长-缩短循环以及降低板状伪足轴向运动[1]。在上皮细胞系Hep-2细胞中,Cytochalasin D通过抑制肌动蛋白聚合,调节病毒转录的晚期和极晚期,停止宿主的转录过程[3]。在感染PCV2的IPEC-J2细胞模型中,给予Cytochalasin D可以抑制PCV2侵袭、复制以及释放,从而抑制病毒入侵[4]。
参考文献:
[1]. Sayyad, W.A., et al., The role of myosin-II in force generation of DRG filopodia and lamellipodia. Sci Rep, 2015. 5: p. 7842.
[2]. Montazeri, M., et al., Activation of Toll-like receptor 3 reduces actin polymerization and adhesion molecule expression in endometrial cells, a potential mechanism for viral-induced implantation failure. Hum Reprod, 2015.
[3]. Volkman, L.E., Baculoviruses and nucleosome management. Virology, 2015. 476c: p. 257-263.
[4]. Yan, M., L. Zhu, and Q. Yang, Infection of porcine circovirus 2 (PCV2) in intestinal porcine epithelial cell line (IPEC-J2) and interaction between PCV2 and IPEC-J2 microfilaments. Virol J, 2014. 11: p. 193
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Physical Appearance | A crystalline solid |
Storage | Desiccate at -20°C |
M.Wt | 507.63 |
Cas No. | 22144-77-0 |
Formula | C30H37NO6 |
Solubility | Soluble in DMSO |
Chemical Name | (3S,3aR,4R,6R,6aS,7E,10R,12S,13Z,15R,15aS)-3-benzyl-6,12-dihydroxy-4,10,12-trimethyl-5-methylene-1,11-dioxo-2,3,3a,4,5,6,6a,9,10,11,12,15-dodecahydro-1H-cycloundeca[d]isoindol-15-yl acetate |
SDF | Download SDF |
Canonical SMILES | O[C@@H]1[C@@H](/C=C/C[C@@H](C)C2=O)[C@]3([C@@H](C=C[C@]2(C)O)OC(C)=O)[C@@H]([C@@H](C)C1=C)[C@H](CC4=CC=CC=C4)NC3=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
细胞实验 [1-3]: | |
细胞系 |
HeLa、Vero、L、HEp2和MDBK细胞,SC-1细胞,小鼠CT26结肠直肠癌细胞 |
溶解方法 |
该化合物在DMSO中的溶解度大于10 mM。若获取更高浓度的溶液,可在37℃下孵育10分钟,随后在超声波浴中摇匀。-20℃以下可储存数月。 |
反应条件 |
0.2–0.5 μg/mL |
应用 |
在HeLa、Vero、L、HEp2和MDBK细胞中,cytochalasin D (0.2–0.5 μg/mL)诱导持续收缩(挛缩)、微绒毛的损失、内质蛋白(zeiosis)的表达、核突出和细胞质过程的延伸。G1期细胞对CD最为敏感;早S期或至少中S期至G2期中,反应性逐渐降低。CD抑制HeLa中[14C]脱氧葡萄糖的转运。在SC-1细胞中,Cytochalasin D处理严重破坏了网络组织,增加了肌动蛋白长丝数目,并导致丝状聚集体或主要由肌动蛋白丝组成的病灶的形成。Cytochalasin D (0.24-15 μg/mL, 16 h)以时间和剂量依赖性方式抑制CT26肿瘤细胞增殖,显著诱导CT26细胞凋亡。 |
动物实验 [3,4]: | |
动物模型 |
小鼠CT26肿瘤模型,猪冠状动脉模型 |
给药剂量 |
静脉注射,50 mg/kg,每3天,持续21天 |
应用 |
静脉注射Cytochalasin D(50 mg/kg)显著抑制肿瘤生长,延长CT26荷瘤小鼠的存活时间。在猪冠状动脉模型中,低剂量Cytochalasin D(2 μg)导致更少的晚期管腔丢失。大剂量Cytochalasin D(20 μg)抑制晚期管腔损失和内膜面积。 |
注意事项 |
由于实验环境的不同,实际溶解度可能与理论值略有不同,请测试室内所有化合物的溶解度。 |
References: [1]. Miranda A F, Godman G C, Deitch A D, et al. Action of cytochalasin D on cells of established lines[J]. The Journal of cell biology, 1974, 61(2): 481-500. [2]. Schliwa M. Action of cytochalasin D on cytoskeletal networks[J]. The Journal of cell biology, 1982, 92(1): 79-91. [3]. Huang F Y, Li Y N, Mei W L, et al. Cytochalasin D, a tropical fungal metabolite, inhibits CT26 tumor growth and angiogenesis[J]. Asian Pacific journal of tropical medicine, 2012, 5(3): 169-174. [4].Salu K J, Bosmans J M, Huang Y, et al. Effects of cytochalasin D-eluting stents on intimal hyperplasia in a porcine coronary artery model[J]. Cardiovascular research, 2006, 69(2): 536-544. |