CA-5f
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
CA-5f是一种有效的晚期巨自噬/自噬(macroautophagy/autophagy)抑制剂，通过抑制自噬体-溶酶体融合起作用。CA-5f能够增加LC3B-II(一种监控自噬的标记物)和SQSTM1蛋白水平，促进ROS的产生。具有抗肿瘤活性。用 CA-5f处理人脐静脉内皮细胞(HUVEC)1小时可抑制细胞骨架蛋白和膜运输蛋白的水平。CA-5f通过增加线粒体衍生的活性氧(ROS)的产生，对A549 NSCLC细胞表现出强烈的细胞毒性，但对正常HUVEC的细胞毒性较低。CA-5f作为单一药物有效抑制A549肺癌异种移植物的生长，在体内具有极好的耐受性[1]。
参考文献：
[1]. Zhang L, Qiang P, Yu J, Miao Y, Chen Z, Qu J, Zhao Q, Chen Z, Liu Y, Yao X, Liu B, Cui L, Jing H, Sun G. Identification of compound CA-5f as a novel late-stage autophagy inhibitor with potent anti-tumor effect against non-small cell lung cancer. Autophagy. 2019 Mar;15(3):391-406. doi: 10.1080/15548627.2018.1511503. Epub 2018 Sep 6. PMID: 30145925; PMCID: PMC6351124.
| Storage | Store at -20°C |
| M.Wt | 388.47 |
| Cas No. | 1370032-19-1 |
| Formula | C24H24N2O3 |
| Solubility | ≥35.4 mg/mL in DMSO |
| Chemical Name | (E)-3-((1H-indol-3-yl)methylene)-5-((E)-3,4-dimethoxybenzylidene)-1-methylpiperidin-4-one |
| SDF | Download SDF |
| Canonical SMILES | O=C(/C(CN(C)C/1)=C/C2=CNC3=C2C=CC=C3)C1=C\C4=CC=C(OC)C(OC)=C4 |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
