BX-912
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
BX-912是３-磷酸肌醇依赖性激酶(3-phosphoinositide-dependent kinase 1，PDK1)的一种有效的、选择性抑制剂，IC50值26 nM[1]。BX-912对PDK1的选择性是PKA的9倍，是PKC的105倍[1]。
已显示BX-912抑制MDA-468细胞(一种表达高水平激活的Akt的乳腺癌肿瘤细胞)的生长，提高Caspase-3/7的活性。然而BX-912不影响正常的原始PrECs和原始HMECs细胞中的Caspase-3/7活性。此外，已证实BX-912能诱导MDA-468细胞的细胞周期停止在G2/M期[1]。
参考文献：
[1] Feldman RI1,Wu JM,Polokoff MA,Kochanny MJ,Dinter H,Zhu D,Biroc SL,Alicke B,Bryant J,Yuan S,Buckman BO,Lentz D,Ferrer M,Whitlow M,Adler M,Finster S,Chang Z,Arnaiz DO. Novel small molecule inhibitors of 3-phosphoinositide-dependent kinase-1. J Biol Chem.2005 May 20;280(20):19867-74. Epub 2005 Mar 16.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 471.35 |
| Cas No. | 702674-56-4 |
| Formula | C20H23BrN8O |
| Solubility | ≥23.55 mg/mL in DMSO; insoluble in H2O; ≥10.25 mg/mL in EtOH |
| Chemical Name | N-[3-[[5-bromo-4-[2-(1H-imidazol-5-yl)ethylamino]pyrimidin-2-yl]amino]phenyl]pyrrolidine-1-carboxamide |
| SDF | Download SDF |
| Canonical SMILES | C1CCN(C1)C(=O)NC2=CC=CC(=C2)NC3=NC=C(C(=N3)NCCC4=CN=CN4)Br |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
| 描述 | BX-912一个有效的、ATP竞争性的PDK1抑制剂，IC50值26 nM。 | |||||
| 靶点 | PDK-1 | |||||
| IC50 | 26 nM | |||||
质量控制和MSDS
- 批次:
化学结构
