BI 6015
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
BI 6015是肝细胞核因子4α的拮抗剂[1]。
肝细胞核因子4α(HNF4α)是一种核受体,调节肠、肝和胰腺β细胞的基因表达[1]。
BI 6015是HNF4α的拮抗剂。BI6015抑制胰岛素启动子。在T6PNE细胞中,BI6015将内源性胰岛素的表达降低50倍。在MIN6和HepG2细胞中,BI6015抑制HNF4α的表达。此外,BI 6015改变HNF4α蛋白酶的敏感性[1]。在T6PNE细胞中,BI 6015减少长链乙酰基肉碱C18、C18:1、C16和C14的水平,增加乙酰肉碱的水平,表明BI6015增加脂肪酸的氧化。在人肝癌细胞系Hep3B中,BI 6015显示出细胞毒性[1]。
在小鼠中,30 mg/kg的BI6015诱导肝脏中HNF4α蛋白的损失,但在肾和肠中没有作用。此外,BI6015以剂量依赖的方式引起脂肪蓄积和脂肪变性及泡状变化[1]。
参考文献:
[1]. Kiselyuk A, Lee SH, Farber-Katz S, et al. HNF4a Antagonists Discovered by a High-Throughput Screen for Modulators of the Human Insulin Promoter. Chem Biol, 2012, 19(7): 806-818.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 331.35 |
Cas No. | 93987-29-2 |
Formula | C15H13N3O4S |
Solubility | Soluble in DMSO |
Chemical Name | 2-methyl-1-((2-methyl-5-nitrophenyl)sulfonyl)-1H-benzo[d]imidazole |
SDF | Download SDF |
Canonical SMILES | O=S(N1C2=CC=CC=C2N=C1C)(C3=CC([N+]([O-])=O)=CC=C3C)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |