AS 101
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Ammonium tri-chloro(dioxoethylene-O,O′-)tellurate (AS-101), an anti–IL-10 compound, is a nontoxic immunomodulator.
AS101 (0.5-2.5 μg/ml) decreased IL-10 production in a dose-dependent manner. Incubation of RMCL and RMC in the presence of AS101 gradually and significantly decreased mesangial cell proliferation [1]. AS101 (0.5 μg/ml, 1 μg/ml) decreased tyrosine phosphorylation and nuclear translocation of stat3 via inhibition of IL-10 [2]. AS-101 induced proliferation and IL-2 production by human lymphocytes and enhanced the production of IL-2 and colony-stimulating factor by mouse spleen cells. Systemically administration of AS-101 to mice mediated antitumour effects. AS-101 directly enhanced the ratio of OKT4 to OKT8-positive cells in cultured mononuclear cells from AIDS patients [3]. AS101 showed significant bone marrow (BM)-sparing effects and preventd hair loss in chemotherapy-treated patients, with minimal overall toxicity [4]. In mice subjected to cecal ligation and puncture (CLP), treatment with AS101 significantly increased survival of septic mice. AS101 treatment restored the ability of these cells to secrete TNF-α and IL-1β. AS101 increased class II Ag expression on peritoneal macrophages. AS101 increased the level of IFN-γ secreted by splenocytes [5].
References:
[1] Kalechman Y, Sredni B, Weinstein T, et al. Production of the novel mesangial autocrine growth factors GDNF and IL-10 is regulated by the immunomodulator AS101[J]. Journal of the American Society of Nephrology, 2003, 14(3): 620-630.
[2] Kalechman Y, Gafter U, Weinstein T, et al. Inhibition of Interleukin-10 by the Immunomodulator AS101 Reduces Mesangial Cell Proliferation in Experimental Mesangioproliferative Glomerulonephritis ASSOCIATION WITH DEPHOSPHORYLATION OF STAT3[J]. Journal of Biological Chemistry, 2004, 279(23): 24724-24732.
[3] Sredni B, Caspi R R, Klein A, et al. A new immunomodulating compound (AS-101) with potential therapeutic application[J]. Nature, 1987, 330(6144): 173-176.
[4] Sredni B, Albeck M, Tichler T, et al. Bone marrow-sparing and prevention of alopecia by AS101 in non-small-cell lung cancer patients treated with carboplatin and etoposide[J]. Journal of Clinical Oncology, 1995, 13(9): 2342-2353.
[5] Kalechman Y, Gafter U, Gal R, et al. Anti-IL-10 therapeutic strategy using the immunomodulator AS101 in protecting mice from sepsis-induced death: dependence on timing of immunomodulating intervention[J]. The Journal of Immunology, 2002, 169(1): 384-392.
Physical Appearance | White crystalline solid |
Storage | Store at -20°C |
M.Wt | 312.05 |
Cas No. | 106566-58-9 |
Formula | C2H4Cl3O2Te·NH4 |
Solubility | Soluble in DMSO |
Chemical Name | Ammonium trichloro[1,2-ethanediolato-O,O']-tellurate |
SDF | Download SDF |
Canonical SMILES | C1CO[Te](O1)(Cl)(Cl)Cl.[NH4+] |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |