Ac-VEID-pNA
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Ac-VEID-pNA 是一种caspase-6的化学显色底物(Km=30µM; Kcat/Km=168’000 M-1sec-1), 也是其他的一些相似的半胱氨酸蛋白酶的底物。能够识别出lamin A切割位点的氨基酸序列VEID。在凋亡过程中，caspase 6 切割底物并释放pNA, 因此caspase的活性可以通过分光光度计检测pNA来决定（at 405nm， ε=10,500 M-1cm-1). 在凋亡细胞的裂解物中，Ac-VEID-pNA 可以用来鉴定和量化caspase-6 的活性，研究caspase-6 被激活后的下游通路。
References:
1. A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis.: N.A. Thornberry et al.; J. Biol. Chem. 272, 17907 (1997)
2. Substrate specificities of caspase family proteases.: R.V. Talanian et al.; J. Biol. Chem. 272, 9677 (1997)
3. The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32.: S.M. Srinivasula et al.; J. Biol. Chem. 271, 27099 (1996)
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 636.65 |
| Cas No. | 189684-54-6 |
| Formula | C28H40N6O11 |
| Solubility | ≥63.7 mg/mL in DMSO; insoluble in EtOH; insoluble in H2O |
| Chemical Name | 10-(sec-butyl)-7-(2-carboxyethyl)-4-isopropyl-13-((4-nitrophenyl)carbamoyl)-2,5,8,11-tetraoxo-3,6,9,12-tetraazapentadecan-15-oic acid |
| SDF | Download SDF |
| Canonical SMILES | O=C(O)CCC(NC(C(NC(C)=O)C(C)C)=O)C(NC(C(C)CC)C(NC(CC(O)=O)C(NC1=CC=C([N+]([O-])=O)C=C1)=O)=O)=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
