Ac-IETD-pNA
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Ac-IETD-pNA是一种caspase-8的化学显色底物，以及一些相似的半胱氨酸蛋白酶的底物(caspase-6, caspase-10 and granzyme B)。它能够辨认出在caspase-3 的前体中的caspase-8切割位点氨基酸序列IETD。在凋亡过程中， caspase 8 切割底物并释放了 pNA, 所以caspase 的活性可以由分光光度计检测pNA来决定（at 405nM）。 在细胞裂解物中，Ac-IETD-pNA能够用于鉴定和量化caspase-8的活性，以及研究caspase-8 被激活后的下游通路 。
References:
1. Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis: D.W. Nicholson, et al.; Nature 376, 37 (1995)
2. Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE: Y.A. Lazebnik, et al.; Nature 371, 346 (1994)
3. Interleukin-1 beta converting enzyme: N.A. Thornberry; Methods Enzymol. 244, 615 (1994)
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 638.62 |
| Cas No. | 219138-21-3 |
| Formula | C27H38N6O12 |
| Solubility | ≥63.9 mg/mL in DMSO; ≥14.03 mg/mL in H2O; ≥5.99 mg/mL in EtOH with ultrasonic |
| Chemical Name | (4S,7S,10S,13S)-4-((S)-sec-butyl)-7-(2-carboxyethyl)-10-((R)-1-hydroxyethyl)-13-((4-nitrophenyl)carbamoyl)-2,5,8,11-tetraoxo-3,6,9,12-tetraazapentadecan-15-oic acid |
| SDF | Download SDF |
| Canonical SMILES | O=C(O)CC[C@H](NC([C@@H](NC(C)=O)[C@@H](C)CC)=O)C(N[C@@H]([C@H](O)C)C(N[C@@H](CC(O)=O)C(NC1=CC=C([N+]([O-])=O)C=C1)=O)=O)=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
质量控制和MSDS
- 批次:
化学结构
