Ac-IETD-AFC
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Ac-IETD-AFC 是一种荧光显色的caspase-8的底物，包含氨基酸序列IETD，是caspase-8传导的蛋白水解的目标。同时也是caspase-3 processing enzyme, caspase-10 和granzyme b的底物。Caspase的活性可以由荧光检测游离的AFC来决定（Ex. = 400 nm，Em. = 505 nm）. 在凋亡的细胞裂解物中，Ac-IETD-AFC还能够用于鉴别和量化caspase 8的活性，用来研究caspase-8被激活后下游的信号通路。
References:
1. Mashima T, Naito M, Kataoka S, Kawai H, Tsuruo T. Aspartate-based inhibitor of interleukin-1 beta-converting enzyme prevents antitumor agent-induced apoptosis in human myeloid leukemia U937 cells. Biochem Biophys Res Commun. 1995; 209(3):907-915.
2. Nicholson DW, Ali A, Thornberry NA, et al. Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. Nature. 1995; 376(6535):17-18.
3. Thornberry NA, Lazebnik Y. Caspases: enemies within. Science. 1998; 281(5381):1312-1316.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 729.65 |
| Cas No. | 211990-57-7 |
| Formula | C31H38F3N5O12 |
| Solubility | ≥73 mg/mL in DMSO; insoluble in H2O; ≥2.84 mg/mL in EtOH with ultrasonic |
| Chemical Name | 4-(sec-butyl)-7-(2-carboxyethyl)-10-(1-hydroxyethyl)-2,5,8,11-tetraoxo-13-((2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl)carbamoyl)-3,6,9,12-tetraazapentadecan-15-oic acid |
| SDF | Download SDF |
| Canonical SMILES | O=C(O)CCC(NC(C(NC(C)=O)C(C)CC)=O)C(NC(C(O)C)C(NC(CC(O)=O)C(NC1=CC(O2)=C(C=C1)C(C(F)(F)F)=CC2=O)=O)=O)=O |
| 运输条件 | 蓝冰运输或根据您的需求运输。 |
| 一般建议 | 不同厂家不同批次产品溶解度各有差异，仅做参考。若实验所需浓度过大至产品溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。 |
