25(S)-27-hydroxy Cholesterol
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
25(S)-27 hydroxy Cholesterol is a stereoisomer of the endogenous oxysterol 27-hydroxy cholesterol. 27-hydroxy Cholesterol is a sterol 27-hydroxylase-mediated cholesterol hydroxylation product involved in activating LXRα and LXRβ and potently suppressing cholesterol biosynthesis [1,2].
Liver X receptors (LXRs) belong to the nuclear receptor superfamily member. LXRs are ligand-activated transcription factors containing two LXR isoforms termed as α and β. LXRs play central roles in the transcriptional control of lipid metabolism. LXRs function as nuclear cholesterol sensors that are activated in response to elevated intracellular cholesterol levels in multiple cell types. Activation of LXRs induces the expression of the genes involved in cholesterol absorption, transport, efflux, and excretion. LXRs are potential targets for a variety of diseases, such as hypertriglyceridemia [3]. LXRs have also been involved in modulating immune and inflammatory responses in macrophages [3].
25(S)-27 hydroxy Cholesterol was the less predominant-occurring (~20%) stereoisomer of the endogenous oxysterol 27-hydroxy cholesterol. 27-hydroxy Cholesterol activated LXRα and LXRβ with the EC50 values of 85 and 71 nM, respectively [1,2].
References:
[1] Fu, X. ,Menke, J.G.,Chen, Y., et al. 27-Hydroxycholesterol is an endogenous ligand for liver X receptor in cholesterol-loaded cells. The Journal of Biological Chemisty 276(42), 38378-38387 (2001).
[2] Javitt, N. B. 25R,26-Hydroxycholesterol revisited: Synthesis, metabolism, and biologic roles. Journal of Lipid Research 43, 665-670 (2002).
[3] Zelcer N, Tontonoz P. Liver X receptors as integrators of metabolic and inflammatory signaling[J]. The Journal of clinical investigation, 2006, 116(3): 607-614.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 402.7 |
Cas No. | 56845-83-1 |
Formula | C27H46O2 |
Synonyms | (25S)-26-hydroxy cholesterol |
Solubility | ≤20mg/ml in ethanol;0.1mg/ml in DMSO;2mg/ml in dimethyl formamide |
Chemical Name | (25S)-cholest-5-ene-3β,26-diol |
SDF | Download SDF |
Canonical SMILES | O[C@@H]1CC2=CC[C@]3([H])[C@@](CC[C@]4([C@@]3([H])CC[C@]4([H])[C@@H](CCC[C@H](C)CO)C)C)([H])[C@](C)2CC1 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |