2-HBA
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
2-HBA is an inducer of the Keap1-Nrf2-ARE pathway.
Keap1-Nrf2-ARE directly react with Keap1, the sensor protein for inducers, leads to enhanced transcription of phase 2 genes and protection against oxidant and electrophile toxicities.
In vitro: 2-HBA could markedly increase the activities of NAD(P)H:quinone acceptor oxidoreductase 1 (NQO1) and glutathione reductase, the levels of total glutathione, as well as the phase 2 response markers. In addition, at high concentrations 2-HBA caused G2/M cell cycle arrest and apoptosis. Moreover, the mutant L1210 cell line was found to be more sensitive to the apoptotic effects of 2-HBA [1].
In vivo: The effect of 2-HBA on the DMBA-induced expression of the Ha-ras gene in isolated RNA tissues of CBA/Ca inbred mice was investigated. According to the previous findings, elevated Ha-ras expression was obserrved even 24 h after DMBA treatment. Administration of 2-HBA with DMBA could lead to a decrease of the DMBA-induced Ha-ras gene expression in all the investigated tissues, suggesting metabolic interaction of 2-HBA and DMBA. In addiiton, administration of 2-HBA 24 h prior to the DMBA treatment was able to reduce the Ha-ras gene expression in all tested tissues except the liver, which could be the result of a possible CYP1A inducer and pro-oxidant effects of 2-HBA [2].
Clinical trial: Up to now, 2-HBA is still in the preclinical development stage.
References:
[1] A. T. Dinkova-Kostova, A. H. Cory, R. E. Bozak, et al. Bis(2-hydroxybenzylidene)acetone, a potent inducer of the phase 2 response, causes apoptosis in mouse leukemia cells through a p53-independent, caspase-mediated pathway. Cancer Letters 245, 341-349 (2007).
[2] Perjési P, Ember I, Bozak RE, Nádasi E, Rozmer Z, Varjas T, Hicks RJ. Effect of the chalcone analog E,E-bis(2-hydroxybenzylidene) acetone on the 7,12-dimethylbenz[a]anthracene-induced Ha-ras gene activity in vivo. In Vivo. 2006 Jan-Feb;20(1):141-6.
Physical Appearance | A crystalline solid |
Storage | Store at -20°C |
M.Wt | 266.3 |
Cas No. | 131359-24-5 |
Formula | C17H14O3 |
Synonyms | Bis(2-hydroxybenzylidene)acetone |
Solubility | ≤14mg/ml in ethanol;11mg/ml in DMSO;12mg/ml in dimethyl formamide |
Chemical Name | (1E,4E)-1,5-bis(2-hydroxyphenyl)-1,4-pentadien-3-one |
SDF | Download SDF |
Canonical SMILES | OC1=C(/C=C/C(/C=C/C2=CC=CC=C2O)=O)C=CC=C1 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
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