2,3-Butanedione-2-monoxime
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
2,3-Butanedione-2-monoxime is a myosin ATPase inhibitor.
Myosin, an ATPase, can convert chemical energy into directed movement and is regarded as a molecular motor. Myosin has various shapes and sizes. More than 11 myosin classes have been identified, and more will be found. The common feature of all of these molecules is a section close to the N terminus, which can be identified as a motor domain.
In vitro: 2,3-Butanedione-2-monoxime (BDM), a general probe of myosin function, was widely used in muscle research as a low-affinity but specific chemical phosphatase that could reversibly inhibit the myosin cross-bridge cycle. It was found that wild-type cells treated with BDM at 20 mM for around two generation times were smaller than untreated controls and showed a septation index about twice that observed in the absence of the inhibitor. Moreover, the organization of actin at the cell poles was disorganized in the presence of BDM, however, cells formed a cytokinetic actin ring. In addition, when nitrogen-starved stationary-phase cells were reinoculated into fresh medium in the presence of BDM, the time taken to repolarize the actin cytoskeleton and to resume the characteristic vegetative cell shape were both delayed substantially [1].
In vivo: So far, there is no animal in vivo data reported.
Clinical trial: So far, no clinical study has been conducted.
Reference:
[1] May KM, Wheatley SP, Amin V, Hyams JS. The myosin ATPase inhibitor 2,3-butanedione-2-monoxime (BDM) inhibits tip growth and cytokinesis in the fission yeast, Schizosaccharomyces pombe. Cell Motil Cytoskeleton. 1998;41(2):117-25.
Storage | Store at -20°C |
M.Wt | 101.1 |
Cas No. | 57-71-6 |
Formula | C4H7NO2 |
Synonyms | BDM|Diacetyl Monoxime|NSC 660|NSC 116103 |
Solubility | ≥5.25 mg/mL in DMSO; ≥60.2 mg/mL in H2O; ≥99.6 mg/mL in EtOH |
Chemical Name | 2,3-butanedione, 2-oxime |
SDF | Download SDF |
Canonical SMILES | CC(/C(C)=N/O)=O |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
- 批次: