VU0810464
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
VU0810464是有效的且选择性的非尿素蛋白门控的内向整流钾通道 (GIRK, Kir3)激活剂。VU0810464对神经元GIRK1/2 (EC50=165 nM)和GIRK1/4 (EC50=720 nM) 神经通道具有纳摩尔效价,并且具有脑部渗透性[1][2]。VU0810464(0、0.1、0.3、1、3、10、30μM)在SAN和HPC细胞中产生浓度依赖性的电流反应曲线,此外,VU0810464对神经元中Kir3通道激活的效力比SAN细胞高9倍。VU0810464对雄性C57BL/6J小鼠产生剂量依赖性的SIH响应降低(腹腔注射;30mg/kg,10mg/kg;30mg/kg;预处理30分钟)。
参考文献:
[1]. Vo BN, Abney KK, Anderson A, Marron Fernandez de Velasco E, Benneyworth MA, Daniels JS, Morrison RD, Hopkins CR, Weaver CD, Wickman K. VU0810464, a non-urea G protein-gated inwardly rectifying K+(Kir3/GIRK) channel activator, exhibits enhanced selectivity for neuronal Kir3 channels and reduces stress-induced hyperthermia in mice. Br J Pharmacol. 2019 Jul;176(13):2238-2249. doi: 10.1111/bph.14671. Epub 2019 May 30. PMID: 30924523; PMCID: PMC6555862.
[2]. Wieting JM, Vadukoot AK, Sharma S, Abney KK, Bridges TM, Daniels JS, Morrison RD, Wickman K, Weaver CD, Hopkins CR. Discovery and Characterization of 1H-Pyrazol-5-yl-2-phenylacetamides as Novel, Non-Urea-Containing GIRK1/2 Potassium Channel Activators. ACS Chem Neurosci. 2017 Sep 20;8(9):1873-1879. doi: 10.1021/acschemneuro.7b00217. Epub 2017 Jul 19. PMID: 28697302; PMCID: PMC6116837.
Storage | Store at -20°C |
M.Wt | 349.83 |
Cas No. | 2126040-21-7 |
Formula | C18H21ClFN3O |
Solubility | ≥46 mg/mL in DMSO |
Chemical Name | 2-(3-chloro-4-fluorophenyl)-N-(1-cyclohexyl-3-methyl-1H-pyrazol-5-yl)acetamide |
SDF | Download SDF |
Canonical SMILES | O=C(CC1=CC=C(C(Cl)=C1)F)NC2=CC(C)=NN2C3CCCCC3 |
运输条件 | 蓝冰运输或根据您的需求运输。 |
一般建议 | 不同厂家不同批次产品溶解度各有差异,仅做参考。若实验所需浓度过大至产品溶解极限,请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存,请尽快用完。 |
质量控制和MSDS
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