TEV Protease
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
TEV蛋白酶是一种具有最高催化效率的高度特异性半胱氨酸蛋白酶。其识别序列为ENLYFQ ▼S，但P1'位置的氨基酸也可以是G、A、M、C或H（1）。TEV蛋白酶通常用于从融合蛋白中去除亲和纯化标签，例如用于去除融合蛋白的Glutathione S-transferase (GST)、His或者其它标签的蛋白酶。TEV蛋白酶具有His标签，可从使用镍亲和树脂的反应中轻松去除，所以适用于带His标签的融合蛋白的酶切反应，而没有完全酶切的带His标签的重组蛋白、切除下来的His标签以及TEV Protease (His-tag)都可以经镍柱结合的方式除去，最终得到的流穿液（Flow-through）中含有所需的靶蛋白。在实际操作过程中，为尽量保留目的蛋白的结构和生物活性，建议在4℃用TEV Protease酶切过夜。TEV Protease在pH6.0-9.0范围内具有活性，而当pH小于或等于5时，会降低甚至失去酶活性。
| Component | 1000 U | 5000 U | 10000 U |
| TEV Protease | 0.1 ml | 0.5 ml | 1 ml |
| 10X TEV Protease Reaction Buffer | 1 ml | 1 ml x5 | 1 ml x10 |
Long-term storage: -20 °C | |||